14-9757-82

antibody from Invitrogen Antibodies
Targeting: ERBB2 CD340, HER-2, HER2, NEU, NGL
Provider product page for 14-9757-82

Antibody data

Product number
14-9757-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
ErbB2 (HER-2) Monoclonal Antibody (MJD2), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This MJD2 monoclonal antibody reacts with HER-2, the product of the ErbB2 gene and a member of the human epidermal growth factor receptor (EGFR) family. HER-2 is a 185 kDa transmembrane receptor with an extracellular binding domain and an intracellular tyrosine kinase domain. Activation of HER-2 occurs following formation of homodimers or heterodimers with ligand-bound EGFR family members, HER3 or HER4. Overexpression of HER-2 results in auto-phosphorylation and increased proliferation and invasiveness and is associated with subsets of multiple cancer types such as breast, ovarian, cervical, uterine, and gastric. The use of HER-2 targeted agents has been used extensively to treat HER-2-positive breast cancer, but a recent study found that these agents can also block the proliferation of breast cancer stem cells found in HER-2-negative patients and suggests the importance of HER-2 as a target for multiple types of breast cancer. The MJD2 antibody recognizes the extracellular domain of HER-2 but at an epitope distinct from that recognized by the 4D5 (Herceptin) and SP3 monoclonal antibodies. Applications Reported: This MJD2 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry. Applications Tested: This MJD2 antibody has been tested by immunhistochemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. This MJD2 antibody has been tested by immunocytochemistry of methanol-fixed and permeabilized human BT474 cells and can be used at less than or equal to 5 µg/mL. This MJD2 antibody has been tested by western blot analysis of reduced human BT474 cell lysate and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
MJD2
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4°C
ERBB2 protein structure - 14-9757-82 shown in red.