PA5-14634

antibody from Invitrogen Antibodies

Targeting: ERBB2 CD340, HER-2, HER2, NEU, NGL

Antibody data

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Validation data

Product number: PA5-14634 - Provider product page
Provider: Invitrogen Antibodies
Product name: ErbB2 (HER-2) Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This antibody is predicted to react with mouse and rat based on sequence homology.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 400 µL
Storage: -20° C, Avoid Freeze/Thaw Cycles

ERBB2 protein structure - PA5-14634 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ERBB2 (arrow) using an ERBB2 polyclonal antibody (Product # PA5-14634) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the ERBB2 gene (Lane 2).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of T47D cell lysates, either noninduced (Lane 1) or induced with HRG (Lane 2) using an ERBB2 polyclonal antibody (Product # PA5-14634).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of SK-BR-3 (Lane 1), T-47D (Lane 2) and MDA-MB-231 (Lane 3). The blot was probed with Anti-ErbB2 Rabbit Polyclonal Antibody (Product # PA5-14634, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 185 kDa band corresponding to ErbB2 was observed in SK-BR-3 and T-47D but not in MDA-MB-231, which is a negative control for ErbB2. Apart from desired band, non specific band was also observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane using the wet transfer system. The membrane was probed with the relevant primary and secondary antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of SK-BR-3 (Lane 1), T-47D (Lane 2) and MDA-MB-231 (Lane 3). The blot was probed with Anti-ErbB2 Rabbit Polyclonal Antibody (Product # PA5-14634, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 185 kDa band corresponding to ErbB2 was observed in SK-BR-3 and T-47D but not in MDA-MB-231, which is a negative control for ErbB2. Apart from desired band, non specific band was also observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane using the wet transfer system. The membrane was probed with the relevant primary and secondary antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).