Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- H00007322-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00007322-M02, RRID:AB_2288011
- Product name
- Mouse Monoclonal UbcH5b/UBE2D2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. UBE2D2 (4A1)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references A 4-gene signature predicts survival of patients with resected adenocarcinoma of the esophagus, junction, and gastric cardia.
Peters CJ, Rees JR, Hardwick RH, Hardwick JS, Vowler SL, Ong CA, Zhang C, Save V, O'Donovan M, Rassl D, Alderson D, Caldas C, Fitzgerald RC, Oesophageal Cancer Clinical and Molecular Stratification (OCCAMS) Study Group.
Gastroenterology 2010 Dec;139(6):1995-2004.e15
Gastroenterology 2010 Dec;139(6):1995-2004.e15
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02] - UBE2D2 monoclonal antibody (M02), clone 4A1 Analysis of UBE2D2 expression in NIH/3T3.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02] - UBE2D2 monoclonal antibody (M02), clone 4A1. Analysis of UBE2D2 expression in PC-12.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02] - Detection limit for recombinant GST tagged UBE2D2 is approximately 3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02] - Analysis of monoclonal antibody to UBE2D2 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.