Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP32868PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti Ki-67 (1200-1250)
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 1200-1250 from Human Ki-67
- Reactivity
- Human, Hamster, Rabbit, Sheep, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-Fixed, Paraffin-Embedded Human breast tumor stained with Ki-67 antibody (5 µg/ml), peroxidase-conjugate and DAB chromogen.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Ki-67 expression in actively growing Jurkat cells: Cells were stained with 0.2 µg of Ki-67 antibody (red) and isotype control (green) and positively stained population was identified using PE conjugated goat anti-rabbit IgG secondary antibody. Cells fixed and permeabilized using ice cold 70% ethanol were used in this intracellular staining protocol.