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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [8]
- Flow cytometry [1]
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- Product number
- TA801577 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA801577, RRID:AB_2625990
- Product name
- Ki67 rat monoclonal antibody, clone OTI5D7 (formerly 5D7)
- Antibody type
- Monoclonal
- Description
- Ki67 rat monoclonal antibody, clone OTI5D7 (formerly 5D7)
- Host
- Rat
- Conjugate
- Unconjugated
- Epitope
- MKI67
- Isotype
- IgG
- Antibody clone number
- OTI5D7
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references Molecular characterization of breast cancer CTCs associated with brain metastasis.
Boral D, Vishnoi M, Liu HN, Yin W, Sprouse ML, Scamardo A, Hong DS, Tan TZ, Thiery JP, Chang JC, Marchetti D
Nature communications 2017 Aug 4;8(1):196
Nature communications 2017 Aug 4;8(1):196
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MKI67 rat monoclonal antibody. (TA801577; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MKI67 rat monoclonal antibody. (TA801577; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MKI67 rat monoclonal antibody. (TA801577; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MKI67 rat monoclonal antibody. (TA801577; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MKI67 rat monoclonal antibody. (TA801577; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MKI67 rat monoclonal antibody. (TA801577; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MKI67 rat monoclonal antibody. (TA801577; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MKI67 rat monoclonal antibody. (TA801577; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of fixed and permeabilized Jurkat cells, using anti-Ki67 antibody (TA801577 at 1:10, red), compare to IgG1 isotype control (Black).
- Validation comment
- FC
