Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoelectron microscopy [1]
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Validation data
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- Product number
- 151202 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#151202, RRID:AB_2566621
- Product name
- Purified anti-mouse/human Ki-67
- Antibody type
- Monoclonal
- Antigen
- E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinant protein.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antibody clone number
- 11F6
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- TE-71 cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml Ki-67 (clone 11F6) in 5% FBS overnight at 4°C, followed by Alexa Fluor® 647 Goat anti-rat IgG (clone Poly4054) for two hours and Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
- Conjugate
- Unconjugated