Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 12-5699-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Ki-67 Monoclonal Antibody (20Raj1), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms of Ki-67 exist, a 345 and 395 kDa form that are expressed in dividing cells. Ki-67 is expressed in all cell types and is detectable during active phases of the cell cycle (G1, S, G2, and mitosis) but is absent from resting cells (G0). During interphase, Ki-67 expression is localized to the nucleus but redistributes to the chromosomes during mitosis and has specifically been found to associate with heterochromatin-bound proteins such as chromobox protein homolog 3 (CBX3). In studies of tumor cells, Ki-67 expression has been used as a marker for determining the fraction of proliferating cells within a given population of tumor cells.
- Conjugate
- Yellow dye
- Antibody clone number
- 20Raj1
- Concentration
- 5 µL/Test
Submitted references Therapeutic Cytokine Inhibition Modulates Activation and Homing Receptors of Peripheral Memory B Cell Subsets in Rheumatoid Arthritis Patients.
In Vitro Evaluation of Toxicant Influences on the Immune System.
Cytokines secreted from bone marrow derived mesenchymal stem cells promote apoptosis and change cell cycle distribution of K562 cell line as clinical agent in cell transplantation.
Streptomyces Levis ABRIINW111 Inhibits SW480 Cells Growth by Apoptosis Induction.
Small-Vessel Vasculopathy Due to Aberrant Autophagy in LAMP-2 Deficiency.
Toll-Like Receptor 1/2 and 5 Ligands Enhance the Expression of Cyclin D1 and D3 and Induce Proliferation in Mantle Cell Lymphoma.
NR2F2 regulates bone marrow-derived mesenchymal stem cell-promoted proliferation of Reh cells.
Interleukin-7 and Toll-like receptor 7 induce synergistic B cell and T cell activation.
Neutrophil depletion impairs natural killer cell maturation, function, and homeostasis.
Characteristics of CD8+ T cell subsets in Chinese patients with chronic HIV infection during initial ART.
Mahmood Z, Schmalzing M, Dörner T, Tony HP, Muhammad K
Frontiers in immunology 2020;11:572475
Frontiers in immunology 2020;11:572475
In Vitro Evaluation of Toxicant Influences on the Immune System.
Kasten-Jolly J, Lawrence DA
Current protocols in toxicology 2020 Jun;84(1):e95
Current protocols in toxicology 2020 Jun;84(1):e95
Cytokines secreted from bone marrow derived mesenchymal stem cells promote apoptosis and change cell cycle distribution of K562 cell line as clinical agent in cell transplantation.
Fathi E, Farahzadi R, Valipour B, Sanaat Z
PloS one 2019;14(4):e0215678
PloS one 2019;14(4):e0215678
Streptomyces Levis ABRIINW111 Inhibits SW480 Cells Growth by Apoptosis Induction.
Faramarzian Azimi Maragheh B, Fatourachi P, Mohammadi SM, Valipour B, Behtari M, Dehnad A, Nozad Charoudeh H
Advanced pharmaceutical bulletin 2018 Nov;8(4):675-682
Advanced pharmaceutical bulletin 2018 Nov;8(4):675-682
Small-Vessel Vasculopathy Due to Aberrant Autophagy in LAMP-2 Deficiency.
Nguyen HT, Noguchi S, Sugie K, Matsuo Y, Nguyen CTH, Koito H, Shiojima I, Nishino I, Tsukaguchi H
Scientific reports 2018 Feb 20;8(1):3326
Scientific reports 2018 Feb 20;8(1):3326
Toll-Like Receptor 1/2 and 5 Ligands Enhance the Expression of Cyclin D1 and D3 and Induce Proliferation in Mantle Cell Lymphoma.
Mastorci K, Muraro E, Pasini E, Furlan C, Sigalotti L, Cinco M, Dolcetti R, Fratta E
PloS one 2016;11(4):e0153823
PloS one 2016;11(4):e0153823
NR2F2 regulates bone marrow-derived mesenchymal stem cell-promoted proliferation of Reh cells.
Zhu N, Wang H, Wei J, Wang B, Shan W, Lai X, Zhao Y, Yu J, Huang H
Molecular medicine reports 2016 Aug;14(2):1351-6
Molecular medicine reports 2016 Aug;14(2):1351-6
Interleukin-7 and Toll-like receptor 7 induce synergistic B cell and T cell activation.
Bikker A, Kruize AA, van der Wurff-Jacobs KM, Peters RP, Kleinjan M, Redegeld F, de Jager W, Lafeber FP, van Roon JA
PloS one 2014;9(4):e94756
PloS one 2014;9(4):e94756
Neutrophil depletion impairs natural killer cell maturation, function, and homeostasis.
Jaeger BN, Donadieu J, Cognet C, Bernat C, Ordoñez-Rueda D, Barlogis V, Mahlaoui N, Fenis A, Narni-Mancinelli E, Beaupain B, Bellanné-Chantelot C, Bajénoff M, Malissen B, Malissen M, Vivier E, Ugolini S
The Journal of experimental medicine 2012 Mar 12;209(3):565-80
The Journal of experimental medicine 2012 Mar 12;209(3):565-80
Characteristics of CD8+ T cell subsets in Chinese patients with chronic HIV infection during initial ART.
Jiao Y, Hua W, Zhang T, Zhang Y, Ji Y, Zhang H, Wu H
AIDS research and therapy 2011 Mar 25;8:15
AIDS research and therapy 2011 Mar 25;8:15
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of unstimulated (left) normal human peripheral blood cells with Anti-Human CD3 PerCP-Cy5-5 (Product # 46-0036-42) and Anti-Human Ki-67. Normal human peripheral blood cells were also stimulated with PHA then stained with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue) or Anti-Human Ki-67 PE (purple). All staining was done using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye