Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 61-5699-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Ki-67 Monoclonal Antibody (20Raj1), PE-eFluor 610, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms of Ki-67 exist, a 345 and 395 kDa form that are expressed in dividing cells. Ki-67 is expressed in all cell types and is detectable during active phases of the cell cycle (G1, S, G2, and mitosis) but is absent from resting cells (G0). During interphase, Ki-67 expression is localized to the nucleus but redistributes to the chromosomes during mitosis and has specifically been found to associate with heterochromatin-bound proteins such as chromobox protein homolog 3 (CBX3). In studies of tumor cells, Ki-67 expression has been used as a marker for determining the fraction of proliferating cells within a given population of tumor cells. This monoclonal antibody 20Raj1 recognizes canine Ki-67. Applications Reported: This 20Raj1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 20Raj1 antibody has been pre-titrated and tested by intracellular staining of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 20Raj1
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Human Liver Memory CD8+ T Cells Use Autophagy for Tissue Residence.
Involvement of TWEAK and the NF-κB signaling pathway in lupus nephritis.
Small-Vessel Vasculopathy Due to Aberrant Autophagy in LAMP-2 Deficiency.
Interleukin-7 and Toll-like receptor 7 induce synergistic B cell and T cell activation.
Characteristics of CD8+ T cell subsets in Chinese patients with chronic HIV infection during initial ART.
Swadling L, Pallett LJ, Diniz MO, Baker JM, Amin OE, Stegmann KA, Burton AR, Schmidt NM, Jeffery-Smith A, Zakeri N, Suveizdyte K, Froghi F, Fusai G, Rosenberg WM, Davidson BR, Schurich A, Simon AK, Maini MK
Cell reports 2020 Jan 21;30(3):687-698.e6
Cell reports 2020 Jan 21;30(3):687-698.e6
Involvement of TWEAK and the NF-κB signaling pathway in lupus nephritis.
Sun F, Teng J, Yu P, Li W, Chang J, Xu H
Experimental and therapeutic medicine 2018 Mar;15(3):2611-2619
Experimental and therapeutic medicine 2018 Mar;15(3):2611-2619
Small-Vessel Vasculopathy Due to Aberrant Autophagy in LAMP-2 Deficiency.
Nguyen HT, Noguchi S, Sugie K, Matsuo Y, Nguyen CTH, Koito H, Shiojima I, Nishino I, Tsukaguchi H
Scientific reports 2018 Feb 20;8(1):3326
Scientific reports 2018 Feb 20;8(1):3326
Interleukin-7 and Toll-like receptor 7 induce synergistic B cell and T cell activation.
Bikker A, Kruize AA, van der Wurff-Jacobs KM, Peters RP, Kleinjan M, Redegeld F, de Jager W, Lafeber FP, van Roon JA
PloS one 2014;9(4):e94756
PloS one 2014;9(4):e94756
Characteristics of CD8+ T cell subsets in Chinese patients with chronic HIV infection during initial ART.
Jiao Y, Hua W, Zhang T, Zhang Y, Ji Y, Zhang H, Wu H
AIDS research and therapy 2011 Mar 25;8:15
AIDS research and therapy 2011 Mar 25;8:15
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular staining of normal human peripheral blood cells stimulated for 2 days with immobilized Anti-Human CD3 (Product # 16-0037-81) with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control PE-eFluor® 610 (Product # 61-4714-82) (left) or Anti-Human Ki-67 PE-eFluor® 610 (right). Staining was performed after fixation with the Foxp3/Transcription Factor Staining Buffers (Product # 00-5523-00) and protocol. Total viable cells were used for analysis as determined by staining with Fixable Viability Dye eFluor® 450 staining (Product # 65-0863-14).