69-5699-41

antibody from Invitrogen Antibodies

Targeting: MKI67 MIB-1, PPP1R105

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

69-5699-41

Provider

Invitrogen Antibodies

Product name

Anti-Ki-67 Monoclonal Antibody (20Raj1), eFluor 506, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms of Ki-67 exist, a 345 and 395 kDa form that are expressed in dividing cells. Ki-67 is expressed in all cell types and is detectable during active phases of the cell cycle (G1, S, G2, and mitosis) but is absent from resting cells (G0). During interphase, Ki-67 expression is localized to the nucleus but redistributes to the chromosomes during mitosis and has specifically been found to associate with heterochromatin-bound proteins such as chromobox protein homolog 3 (CBX3). In studies of tumor cells, Ki-67 expression has been used as a marker for determining the fraction of proliferating cells within a given population of tumor cells. This monoclonal antibody 20Raj1 recognizes canine Ki-67. Applications Reported: This 20Raj1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 20Raj1 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

20Raj1

Vial size

25 Tests

Concentration

5 µL/Test

Storage

4° C, store in dark, DO NOT FREEZE!

References

Submitted references

Small-Vessel Vasculopathy Due to Aberrant Autophagy in LAMP-2 Deficiency.

Nguyen HT, Noguchi S, Sugie K, Matsuo Y, Nguyen CTH, Koito H, Shiojima I, Nishino I, Tsukaguchi H
Scientific reports 2018 Feb 20;8(1):3326

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Core Transcription Factors, MicroRNAs, and Small Molecules Drive Transdifferentiation of Human Fibroblasts Towards The Cardiac Cell Lineage.

Christoforou N, Chakraborty S, Kirkton RD, Adler AF, Addis RC, Leong KW
Scientific reports 2017 Jan 10;7:40285

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Interleukin-7 and Toll-like receptor 7 induce synergistic B cell and T cell activation.

Bikker A, Kruize AA, van der Wurff-Jacobs KM, Peters RP, Kleinjan M, Redegeld F, de Jager W, Lafeber FP, van Roon JA
PloS one 2014;9(4):e94756

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Characteristics of CD8+ T cell subsets in Chinese patients with chronic HIV infection during initial ART.

Jiao Y, Hua W, Zhang T, Zhang Y, Ji Y, Zhang H, Wu H
AIDS research and therapy 2011 Mar 25;8:15

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Intracellular staining of unstimulated (left) or Anti-Human CD3 Functional Grade Purified (Product # 16-0037-81)-stimulated (right) normal human peripheral blood cells with Anti-Human CD19 PE (Product # 12-0198-42) and Anti-Human Ki-67 eFluor® 506 (right), using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Total viable cells, as determined by Fixable Viability Dye eFluor® 660 (Product # 65-0864-14), were used for analysis.