HPA001164

MKI67 antibody from Atlas Antibodies

MIB-, PPP1R105

Eligible for validation within the Antibodypedia Validation Initiative

Immunocytochemistry

Supportive data in Antibodypedia

Immunohistochemistry

Enhanced validation

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Antibody Data

Product number

HPA001164

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA001164, RRID:AB_611578

Product name

Anti-MKI67

Provider product page

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

DGPHFPLSLSTCLFGRGIECDIRIQLPVVSKQHCK
IEIHEQEAILHNFSSTNPTQVNGSVIDEPVRLKHG
DVITIIDRSFRYENESLQSGRKSTEFPRKIREQEP
ARRVSRSSFSSDPDEKAQDSKAYSKITEGKVSGNP
QVHI


Isotype

IgG

Vial size

100 µl

Storage

Store at +4¡C for short term storage. Long time storage is recommended at -20¡C.

References

IL-4 induces proliferation in prostate cancer PC3 cells under nutrient-depletion stress through the activation of the JNK-pathway and survivin upregulation
Roca H, Craig M, Ying C, Varsos Z, Czarnieski P, Alva A, Hernandez J, Fuller D, Daignault S, Healy P, Pienta K
Journal of Cellular Biochemistry 2011

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & nuclear bodies.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node shows moderate nucleolar/ nuclear positivity in germinal center cells and non-germinal center cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human bone marrow shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows low expression as expected.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human bone marrow and cerebral cortex tissues using Anti-MKI67 antibody. Corresponding MKI67 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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