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Western blot
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Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [3]
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- Product number
- LS-C674410 - Provider product page
- Provider
- LSBio
- Product name
- HIST1H4I Antibody LS-C674410
- Antibody type
- Polyclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Positive Western Blot detected in Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, NIH/3T3 whole cell lysate. All lanes: HIST1H4A antibody at 0.6 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 12 KDa. Observed band size: 12 KDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating HIST1H4A in Hela whole cell lysate Lane 1: Rabbit control IgG instead of HIST1H4A (Ab-5) Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: HIST1H4A (Ab-5) Antibody (5µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating HIST1H4A in Hela whole cell lysate Lane 1: Rabbit control IgG instead of HIST1H4A (Ab-5) Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: HIST1H4A (Ab-5) Antibody (5µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating HIST1H4A in Hela whole cell lysate Lane 1: Rabbit control IgG instead of HIST1H4A (Ab-5) Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: HIST1H4A (Ab-5) Antibody (5µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)
