Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB100-2128 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2128, RRID:AB_2139399
- Product name
- Rabbit Polyclonal MAML3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MAML3 Antibody [NB100-2128] - Detection of Human and Mouse MAML3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAML3 antibody NB100-2128 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MAML3 Antibody [NB100-2128] - Detection of Human and Mouse MAML3 on HeLa whole cell lysate using NB100-2128. MAML3 was also immunoprecipitated by rabbit anti-MAML3 antibodies NB100-2129 and another MAML3 antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MAML3 Antibody [NB100-2128] - Detection of human MAML3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAML3 antibody NB100-2128 used for IP at 6 ug per reaction. MAML3 was also immunoprecipitated by rabbit anti-MAML3 antibody NB100-2129. For blotting immunoprecipitated MAML3, NB100-2128 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: MAML3 Antibody [NB100-2128] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MAML3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: MAML3 Antibody [NB100-2128] - Detection of human MAML3 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MAML3 (NB100-2128). Detection: DAB