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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [2]
- Other assay [2]
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- Product number
- PA5-13678 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAML3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Long Non-Coding RNA LINC02802 Regulates In Vitro Sprouting Angiogenesis by Sponging microRNA-486-5p.
Notch-dependent RBPJκ inhibits proliferation of human cytotrophoblasts and their differentiation into extravillous trophoblasts.
Rosano S, Parab S, Noghero A, Corà D, Bussolino F
International journal of molecular sciences 2022 Jan 31;23(3)
International journal of molecular sciences 2022 Jan 31;23(3)
Notch-dependent RBPJκ inhibits proliferation of human cytotrophoblasts and their differentiation into extravillous trophoblasts.
Velicky P, Haider S, Otti GR, Fiala C, Pollheimer J, Knöfler M
Molecular human reproduction 2014 Aug;20(8):756-66
Molecular human reproduction 2014 Aug;20(8):756-66
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MAML3 in formalin fixed and paraffin embedded human lymph node. Samples were incubated with MAML3 polyclonal antibody (Product # PA5-13678) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HeLa cells using a MAML3 polyclonal antibody (Product # PA5-13678) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of MAML3 in Hela cells (right histogram). Samples were incubated with MAML3 polyclonal antibody (Product # PA5-13678) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 miR-486-5p directly targets MAML3 ( A ) RT-qPCR analysis of MAML3 in spheroids. Data are represented as mean +- SEM from n = 3 experiments; ****, p < 0.0001. ( B ) Detection of MAML 3 by Western blot analysis. Image are representative of three different experiments. ( C ) Representation of the two binding sites for miR-486-5p on MAML3 3'-UTR. Nucleotides that were mutated to disrupt the interaction are indicated in red. ( D ) Luciferase reporter assay in ECs co-transfected with miR-486-5p mimic together with pcDNA3.1 MAML3 WT or the mutant form. Luciferase signal was assayed at 24 h post-transfection and normalized to Renilla luciferase activity. Data are represented as mean +- SEM from n = 3 experiments; ****, p < 0.0001, ns, not significant. ( E ) RT-qPCR and Western blot analysis of MAML3 expression in ECs transfected with miR-486-5p mimic. ( F ) RT-qPCR and Western blot analysis of MAML3 expression in ECs transfected with miR-486-5p inhibitor. Data are represented as mean +- SEM from n = 3 experiments; ****, p < 0.0001. Western images are representative of three different experiments.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 LINC02802 indirectly modulates MAML3 expression ( A ) RT-qPCR and Western blot analysis of relative MAML3 expression in ECs transfected with LINC02802 wild type or the mutated one. ( B ) RT-qPCR and Western blot analysis of relative MAML3 expression in ECs transfected with si_LINC02802. Data are represented as mean +- SEM from n = 3 experiments; **, p < 0.01; *, p < 0.05; ns, not significant. Blots are representative of three experiments performed with similar results.
