PA5-85625

antibody from Invitrogen Antibodies

Targeting: SSTR3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-85625

Provider

Invitrogen Antibodies

Product name

SSTR3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Keep as concentrated solution.

Concentration

0.54 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SSTR3 in various tissue extracts using SSTR3 polyclonal antibody (Product # PA5-85625) using 50 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SSTR3 in U87-MG whole cell and membrane extract using SSTR3 polyclonal antibody (Product # PA5-85625) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SSTR3 Polyclonal Antibody (Product # PA5-85625). U87-MG whole cell and membrane extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SSTR3 antibody SSTR3 Polyclonal Antibody (Product # PA5-85625) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SSTR3 Polyclonal Antibody (Product # PA5-85625). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SSTR3 Polyclonal Antibody (Product # PA5-85625) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SSTR3 was performed in DIV10 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SSTR3 Polyclonal Antibody (Product # PA5-85625) diluted at 1:500. Red: alpha Tubulin, stained by alpha Tubulin antibody. Blue: Fluoroshield with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of SSTR3 was performed in paraffin-embedded mouse brain tissue using SSTR3 Polyclonal Antibody (Product # PA5-85625) at a dilution of 1:500.