Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-21803 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZNF707 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZNF707 was performed by loading 15 µg of HepG2 whole cell lysate (lanes 1 and 2) or mouse liver whole tissue lysate (lanes 3 and 4) in duplicate onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% milk in PBST for 1 hour at room temperature. The membrane was probed with a ZNF707 polyclonal antibody (Product # PA5-21803) at a dilution of 1:1000 overnight at 4°C, washed in PBST, and probed with an HRP-conjugated anti-rabbit IgG secondary antibody at a dilution of 1:5000 for 1 hour at room temperature. Detection was performed using ECL substrate. NOTE: In HepG2 lysates, ZNF707 was detected around 43 kD, and it was detected just below 40 kD in mouse liver lysates. Data courtesy of the Innovators Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ZNF707 Polyclonal Antibody (Product # PA5-21803). Sample (30 µg of whole cell lysate). Lane A: Hep G2. 10% SDS PAGE. ZNF707 Polyclonal Antibody (Product # PA5-21803) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H3347 xenograft , using ZNF707 (Product # PA5-21803) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.