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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- 703052 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C17orf96 Recombinant Rabbit Monoclonal Antibody (5H7L8)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 5H7L8
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- For immunofluorescence analysis, HEK-293 cells were fixed and permeabilized for detection of endogenous C17orf96 using Anti-C17orf96 Recombinant Rabbit Monoclonal Antibody (Product # 703052, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of C17orf96 protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of C17orf96. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Enrichment of endogenous C17ORF96 protein at specific gene loci using Anti-C17ORF96 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-C17ORF96 Recombinant Rabbit Monoclonal Antibody (Product # 703052, 5 µg) on sheared chromatin from 2 million HEK-293 cells using the MAGnify ChIP System kit (Product # 492024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over Promoters of HOXA9, MYT1, SOX2 and SAT2 satellite repeats . Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
