PA5-112503

antibody from Invitrogen Antibodies

Targeting: H3C7 H3/i, H3FI, HIST1H3F

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-112503

Provider

Invitrogen Antibodies

Product name

beta-hydroxybutyryl-Histone H3 (Lys9) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

0.32 mg/mL

Storage

-20°C or -80°C if preferred

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemichal analysis of beta-hydroxybutyryl Histone H3 (Lys9) in Hela cells using a Polyclonal antibody (Product # PA5-112503) at a dilution of 1:15. Hela cells (treated with 50mM sodium 3-hydroxybutyrate for 72h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemichal analysis of beta-hydroxybutyryl Histone H3 (Lys9) in Hela cells using a Polyclonal antibody (Product # PA5-112503) at a dilution of 1:15. Hela cells (treated with 50mM sodium 3-hydroxybutyrate for 72h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin Immunoprecipitation analysis of beta-hydroxybutyryl Histone H3 (Lys9) in 4*10^6 Hela cells using a beta-hydroxybutyryl Histone H3 (Lys9) polyclonal antibody (Product #PA5-112503). Hela cells were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5 µg anti-HIST1H3A or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.