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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- 39763 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Histone H3 Monoclonal Antibody (MABI 0301)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- MABI 0301
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H3 antibody (mAb) tested by Western blot. HeLa nuclear extract (20 µg per lane) probed with Histone H3 antibody (mab) at a dilution of 2 µg/mL. Lane 1: Nuclear extract of untreated HeLa cells. Lane 2: Nuclear extract of HeLa cells treated with sodium butyrate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H3 antibody (mAb) tested by immunofluorescence. HeLa cells stained with Histone H3 antibody (mAb) at a dilution of 1 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H3 antibody (mAb) tested by ChIP. ChIP performed on Active Motif Ready-to-ChIP HeLa cell chromatin (Catalog No. 53015) using Histone H3 antibody (mAb). PCR was performed using primers specific for the promoter region of the human GAPDH gene. Lane 1: ChIP using mouse IgG as a negative control. Lane 2: ChIP using 10 µg of Histone H3 antibody (mAb). Lane 3: Input DNA control.
