61663

antibody from Invitrogen Antibodies

Targeting: H3C7 H3/i, H3FI, HIST1H3F

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

61663

Provider

Invitrogen Antibodies

Product name

Histone H3K9ac Monoclonal Antibody (2G1F9)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rat

Isotype

IgG

Antibody clone number

2G1F9

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Histone H3K9ac antibody (mAb) (Clone 2G1F9) tested by Western blot. HeLa nuclear extract (20 µg per lane) probed with Histone H3K9ac antibody (mAb) (1 µg/ml dilution). Lane 1: untreated cells. Lane 2: cells treated with sodium butyrate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Histone H3K9ac antibody (mAb) (Clone 2G1F9) tested by immunofluorescence. Left: HeLa cell stained with H3.1 / 3.2 antibody (mAb). Right: Hoechst.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Histone H3K9ac antibody (mAb) (Clone 2G1F9) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 15 µg of chromatin from a human medulloblastoma cell line and 4 µg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 6 million sequence tags were mapped to identify Histone H3K9ac binding sites. The image shows binding across a region of chromosome 22. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.