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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- 91336ACTMOTIF - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Histone H3K9me2 Recombinant Rabbit Monoclonal Antibody, AbFlex®
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AbFlex Histone H3K9me2 antibody tested by Western blot. 20 µg of HeLa nuclear extract was run on SDS-PAGE and probed with antibody at 0.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AbFlex Histone H3K9me2 antibody (rAb) tested by Luminex bead-based specificity analysis. Luminex bead-based specificity analysis was used to confirm the specificity of AbFlex Histone H3K9me2 antibody (rAb) antibody for methyl-lysine 9 H3. Peptides corresponding to regions around major sites of histone H3 methylation or other methyl-lysine peptides were conjugated to MagPlex Luminex beads and incubated with various amounts of AbFlexHistone H3K9me2 antibody (rAb). Peptide-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. Luminex is a registered trademark of Luminex Corporation.
