PA5-97639

antibody from Invitrogen Antibodies

Targeting: CTCFL BORIS, CT27, dJ579F20.2

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-97639

Provider

Invitrogen Antibodies

Product name

CTCFL Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C or -80°C if preferred

References

Submitted references

CTCF Expression and Dynamic Motif Accessibility Modulates Epithelial-Mesenchymal Gene Expression.

Johnson KS, Hussein S, Chakraborty P, Muruganantham A, Mikhail S, Gonzalez G, Song S, Jolly MK, Toneff MJ, Benton ML, Lin YC, Taube JH
Cancers 2022 Jan 1;14(1)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 EMT-driven TFBS accessibility and CTCF expression dynamics. ( A ) Motif enrichment by percentage of 50-bp targets containing motif of the top 20 differentially enriched motifs. ( B ) Motif enrichment percentage change, compared to 0 d, of top 20 differentially enriched motifs. ( C ) The overlap between ATAC-seq peaks (in total, or with CTCF motifs) and CTCF ChIP-seq peaks from Fritz et al. [] was compared to a random distribution model. The observed amount of base pair overlap between the all ATAC-seq peaks and ChIP-seq peaks is 2,758,444 bp, whereas the expected amount of overlap based on our distribution is 110,000.50 bp (s.d. = 4894.19 bp). For the ATAC peaks with a CTCF binding motif, the enrichment is greater (f.c. = 143, p = 0.001). We observe 1,728,041 bp, but expect 12,083.21 bp with a randomly distributed set of genomic regions (s.d. = 1607.12 bp) One thousand permutations were used to generate a p -value, which was corrected using Bonferroni method. * p