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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [44]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MA1-34759 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PMEL Monoclonal Antibody (HMB45)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody reacts with an antigen present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells.This antibody reacts with melanomas and tumors showing melanoma/melanocytic differentiation. It reacts with junctional and blue nevus cells. Non-melanocytic cells do not react with this antibody.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HMB45
- Vial size
- 500 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, do not freeze
Submitted references Two novel TSC2 mutations in renal epithelioid angiomyolipoma sensitive to everolimus.
High fat diet increases melanoma cell growth in the bone marrow by inducing osteopontin and interleukin 6.
A challenging case of ocular melanoma.
Targeting histone deacetylase 6 mediates a dual anti-melanoma effect: Enhanced antitumor immunity and impaired cell proliferation.
ASPL-TFE3 translocation in vulvovaginal alveolar soft part sarcoma.
Critical residues in the PMEL/Pmel17 N-terminus direct the hierarchical assembly of melanosomal fibrils.
Cardiac Epithelioid PEComa: Report of Two Cases and Review of the Literature.
Targeting BRAFV600E in an inducible murine model of melanoma.
Genetic ablation of SOX18 function suppresses tumor lymphangiogenesis and metastasis of melanoma in mice.
Cellular and clinical report of new Griscelli syndrome type III cases.
Effect of PUVA therapy on melanocytes and keratinocytes in non-segmental vitiligo: histopathological, immuno-histochemical and ultrastructural study.
Differential recognition of a dileucine-based sorting signal by AP-1 and AP-3 reveals a requirement for both BLOC-1 and AP-3 in delivery of OCA2 to melanosomes.
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
Mutations in or near the transmembrane domain alter PMEL amyloid formation from functional to pathogenic.
Inactivation of Pmel alters melanosome shape but has only a subtle effect on visible pigmentation.
The recycling endosome protein Rab17 regulates melanocytic filopodia formation and melanosome trafficking.
Proprotein convertases process Pmel17 during secretion.
Microscopic uterine lymphangioleiomyomatosis perivascular epithelioid cell neoplasm: a case report with the earliest manifestation of this enigmatic neoplasm.
Endoscopic ultrasound-guided fine-needle aspiration of intrathoracic and intra-abdominal spindle cell and mesenchymal lesions.
Primary myoepithelial carcinoma of the larynx: case report and review of the literature.
ESCRT-I function is required for Tyrp1 transport from early endosomes to the melanosome limiting membrane.
Patterns of repigmentation in two cases of hypopigmented type of vitiligo.
Clinical and cellular characterisation of Hermansky-Pudlak syndrome type 6.
N-terminal domains elicit formation of functional Pmel17 amyloid fibrils.
Pulmonary meningothelial-like nodules: new insights into a common but poorly understood entity.
Cutaneous metastases of malignant melanoma--how difficult can it be?
Melanocytes expressing MC1R polymorphisms associated with red hair color have altered MSH-ligand activated pigmentary responses in coculture with keratinocytes.
A novel missense mutation (G43S) in the switch I region of Rab27A causing Griscelli syndrome.
Glycolipid-dependent sorting of melanosomal from lysosomal membrane proteins by lumenal determinants.
Vacuolar-type H(+)-ATPase with the a3 isoform is the proton pump on premature melanosomes.
Rab7 regulates maturation of melanosomal matrix protein gp100/Pmel17/Silv.
Cell-specific ATP7A transport sustains copper-dependent tyrosinase activity in melanosomes.
Melanosis in a Holstein heifer.
Cellular defects in Chediak-Higashi syndrome correlate with the molecular genotype and clinical phenotype.
Characterization of t(6;11)(p21;q12) in a renal-cell carcinoma of an adult patient.
BLOC-1 is required for cargo-specific sorting from vacuolar early endosomes toward lysosome-related organelles.
Improper trafficking of melanocyte-specific proteins in Hermansky-Pudlak syndrome type-5.
Dual loss of ER export and endocytic signals with altered melanosome morphology in the silver mutation of Pmel17.
Accumulation of low-avidity anti-melanocortin receptor 1 (anti-MC1R) CD8+ T cells in the lesional skin of a patient with melanoma-related depigmentation.
Mannose receptor targeting of tumor antigen pmel17 to human dendritic cells directs anti-melanoma T cell responses via multiple HLA molecules.
Mannose receptor targeting of tumor antigen pmel17 to human dendritic cells directs anti-melanoma T cell responses via multiple HLA molecules.
HER-2, gp100, and MAGE-1 are expressed in human glioblastoma and recognized by cytotoxic T cells.
A novel splice variant of Pmel17 expressed by human melanocytes and melanoma cells lacking some of the internal repeats.
Proprotein convertase cleavage liberates a fibrillogenic fragment of a resident glycoprotein to initiate melanosome biogenesis.
Wang T, Xie S, Luo R, Shi L, Bai P, Wang X, Wan R, Deng J, Wu Z, Li W, Xiao W, Wang Y, Chen B, Zhang K, Xing J
Cancer biology & therapy 2020;21(1):4-11
Cancer biology & therapy 2020;21(1):4-11
High fat diet increases melanoma cell growth in the bone marrow by inducing osteopontin and interleukin 6.
Chen GL, Luo Y, Eriksson D, Meng X, Qian C, Bäuerle T, Chen XX, Schett G, Bozec A
Oncotarget 2016 May 3;7(18):26653-69
Oncotarget 2016 May 3;7(18):26653-69
A challenging case of ocular melanoma.
Costache M, Dumitru AV, Pătraşcu OM, Popa-Cherecheanu DA, Bădilă P, Miu JC, Procop A, Popa M, Tampa MŞ, Sajin M, Simionescu O, Cîrstoiu MM
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2015;56(2 Suppl):817-22
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2015;56(2 Suppl):817-22
Targeting histone deacetylase 6 mediates a dual anti-melanoma effect: Enhanced antitumor immunity and impaired cell proliferation.
Woan KV, Lienlaf M, Perez-Villaroel P, Lee C, Cheng F, Knox T, Woods DM, Barrios K, Powers J, Sahakian E, Wang HW, Canales J, Marante D, Smalley KSM, Bergman J, Seto E, Kozikowski A, Pinilla-Ibarz J, Sarnaik A, Celis E, Weber J, Sotomayor EM, Villagra A
Molecular oncology 2015 Aug;9(7):1447-1457
Molecular oncology 2015 Aug;9(7):1447-1457
ASPL-TFE3 translocation in vulvovaginal alveolar soft part sarcoma.
Jabbour MN, Seoud M, Al-Ahmadie H, Abdul-Karim FW, Zaatari GS
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2014 May;33(3):263-7
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2014 May;33(3):263-7
Critical residues in the PMEL/Pmel17 N-terminus direct the hierarchical assembly of melanosomal fibrils.
Leonhardt RM, Vigneron N, Hee JS, Graham M, Cresswell P
Molecular biology of the cell 2013 Apr;24(7):964-81
Molecular biology of the cell 2013 Apr;24(7):964-81
Cardiac Epithelioid PEComa: Report of Two Cases and Review of the Literature.
Niu H, Wang FW, Zhang PJ, Bing Z
Case reports in medicine 2012;2012:521678
Case reports in medicine 2012;2012:521678
Targeting BRAFV600E in an inducible murine model of melanoma.
Hooijkaas AI, Gadiot J, van der Valk M, Mooi WJ, Blank CU
The American journal of pathology 2012 Sep;181(3):785-94
The American journal of pathology 2012 Sep;181(3):785-94
Genetic ablation of SOX18 function suppresses tumor lymphangiogenesis and metastasis of melanoma in mice.
Duong T, Proulx ST, Luciani P, Leroux JC, Detmar M, Koopman P, Francois M
Cancer research 2012 Jun 15;72(12):3105-14
Cancer research 2012 Jun 15;72(12):3105-14
Cellular and clinical report of new Griscelli syndrome type III cases.
Westbroek W, Klar A, Cullinane AR, Ziegler SG, Hurvitz H, Ganem A, Wilson K, Dorward H, Huizing M, Tamimi H, Vainshtein I, Berkun Y, Lavie M, Gahl WA, Anikster Y
Pigment cell & melanoma research 2012 Jan;25(1):47-56
Pigment cell & melanoma research 2012 Jan;25(1):47-56
Effect of PUVA therapy on melanocytes and keratinocytes in non-segmental vitiligo: histopathological, immuno-histochemical and ultrastructural study.
Anbar TS, El-Sawy AE, Attia SK, Barakat MT, Moftah NH, El-Ammawy TS, Abdel-Rahman AT, El-Tonsy MH
Photodermatology, photoimmunology & photomedicine 2012 Feb;28(1):17-25
Photodermatology, photoimmunology & photomedicine 2012 Feb;28(1):17-25
Differential recognition of a dileucine-based sorting signal by AP-1 and AP-3 reveals a requirement for both BLOC-1 and AP-3 in delivery of OCA2 to melanosomes.
Sitaram A, Dennis MK, Chaudhuri R, De Jesus-Rojas W, Tenza D, Setty SR, Wood CS, Sviderskaya EV, Bennett DC, Raposo G, Bonifacino JS, Marks MS
Molecular biology of the cell 2012 Aug;23(16):3178-92
Molecular biology of the cell 2012 Aug;23(16):3178-92
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
Yin WH, Li J, Chan JK
Diagnostic pathology 2012 Apr 12;7:39
Diagnostic pathology 2012 Apr 12;7:39
Mutations in or near the transmembrane domain alter PMEL amyloid formation from functional to pathogenic.
Watt B, Tenza D, Lemmon MA, Kerje S, Raposo G, Andersson L, Marks MS
PLoS genetics 2011 Sep;7(9):e1002286
PLoS genetics 2011 Sep;7(9):e1002286
Inactivation of Pmel alters melanosome shape but has only a subtle effect on visible pigmentation.
Hellström AR, Watt B, Fard SS, Tenza D, Mannström P, Narfström K, Ekesten B, Ito S, Wakamatsu K, Larsson J, Ulfendahl M, Kullander K, Raposo G, Kerje S, Hallböök F, Marks MS, Andersson L
PLoS genetics 2011 Sep;7(9):e1002285
PLoS genetics 2011 Sep;7(9):e1002285
The recycling endosome protein Rab17 regulates melanocytic filopodia formation and melanosome trafficking.
Beaumont KA, Hamilton NA, Moores MT, Brown DL, Ohbayashi N, Cairncross O, Cook AL, Smith AG, Misaki R, Fukuda M, Taguchi T, Sturm RA, Stow JL
Traffic (Copenhagen, Denmark) 2011 May;12(5):627-43
Traffic (Copenhagen, Denmark) 2011 May;12(5):627-43
Proprotein convertases process Pmel17 during secretion.
Leonhardt RM, Vigneron N, Rahner C, Cresswell P
The Journal of biological chemistry 2011 Mar 18;286(11):9321-37
The Journal of biological chemistry 2011 Mar 18;286(11):9321-37
Microscopic uterine lymphangioleiomyomatosis perivascular epithelioid cell neoplasm: a case report with the earliest manifestation of this enigmatic neoplasm.
Clay MR, Gibson P, Lowell J, Cooper K
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2011 Jan;30(1):71-5
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2011 Jan;30(1):71-5
Endoscopic ultrasound-guided fine-needle aspiration of intrathoracic and intra-abdominal spindle cell and mesenchymal lesions.
Bean SM, Baker A, Eloubeidi M, Eltoum I, Jhala N, Crowe R, Jhala D, Chhieng DC
Cancer cytopathology 2011 Feb 25;119(1):37-48
Cancer cytopathology 2011 Feb 25;119(1):37-48
Primary myoepithelial carcinoma of the larynx: case report and review of the literature.
Yu G, Qu G, Kong L, Pan X, Wang W, Lv J
Pathology, research and practice 2011 Feb 15;207(2):127-30
Pathology, research and practice 2011 Feb 15;207(2):127-30
ESCRT-I function is required for Tyrp1 transport from early endosomes to the melanosome limiting membrane.
Truschel ST, Simoes S, Setty SR, Harper DC, Tenza D, Thomas PC, Herman KE, Sackett SD, Cowan DC, Theos AC, Raposo G, Marks MS
Traffic (Copenhagen, Denmark) 2009 Sep;10(9):1318-36
Traffic (Copenhagen, Denmark) 2009 Sep;10(9):1318-36
Patterns of repigmentation in two cases of hypopigmented type of vitiligo.
Anbar TS, El-Sawy AE, Attia SK, Moftah NH, El-Tonsy MH
Photodermatology, photoimmunology & photomedicine 2009 Jun;25(3):156-8
Photodermatology, photoimmunology & photomedicine 2009 Jun;25(3):156-8
Clinical and cellular characterisation of Hermansky-Pudlak syndrome type 6.
Huizing M, Pederson B, Hess RA, Griffin A, Helip-Wooley A, Westbroek W, Dorward H, O'Brien KJ, Golas G, Tsilou E, White JG, Gahl WA
Journal of medical genetics 2009 Dec;46(12):803-10
Journal of medical genetics 2009 Dec;46(12):803-10
N-terminal domains elicit formation of functional Pmel17 amyloid fibrils.
Watt B, van Niel G, Fowler DM, Hurbain I, Luk KC, Stayrook SE, Lemmon MA, Raposo G, Shorter J, Kelly JW, Marks MS
The Journal of biological chemistry 2009 Dec 18;284(51):35543-55
The Journal of biological chemistry 2009 Dec 18;284(51):35543-55
Pulmonary meningothelial-like nodules: new insights into a common but poorly understood entity.
Mukhopadhyay S, El-Zammar OA, Katzenstein AL
The American journal of surgical pathology 2009 Apr;33(4):487-95
The American journal of surgical pathology 2009 Apr;33(4):487-95
Cutaneous metastases of malignant melanoma--how difficult can it be?
Zurac S, Andrei R, Petsakos G, Nichita L, Bastian A, Micu G, Gramadă E, Popp C, Stăniceanu F, Petrescu S, Negroiu G, Giurcăneanu D, Chiţu V
Romanian journal of internal medicine = Revue roumaine de medecine interne 2008;46(4):375-8
Romanian journal of internal medicine = Revue roumaine de medecine interne 2008;46(4):375-8
Melanocytes expressing MC1R polymorphisms associated with red hair color have altered MSH-ligand activated pigmentary responses in coculture with keratinocytes.
Roberts DW, Newton RA, Leonard JH, Sturm RA
Journal of cellular physiology 2008 May;215(2):344-55
Journal of cellular physiology 2008 May;215(2):344-55
A novel missense mutation (G43S) in the switch I region of Rab27A causing Griscelli syndrome.
Westbroek W, Tuchman M, Tinloy B, De Wever O, Vilboux T, Hertz JM, Hasle H, Heilmann C, Helip-Wooley A, Kleta R, Gahl WA
Molecular genetics and metabolism 2008 Jun;94(2):248-54
Molecular genetics and metabolism 2008 Jun;94(2):248-54
Glycolipid-dependent sorting of melanosomal from lysosomal membrane proteins by lumenal determinants.
Groux-Degroote S, van Dijk SM, Wolthoorn J, Neumann S, Theos AC, De Mazière AM, Klumperman J, van Meer G, Sprong H
Traffic (Copenhagen, Denmark) 2008 Jun;9(6):951-63
Traffic (Copenhagen, Denmark) 2008 Jun;9(6):951-63
Vacuolar-type H(+)-ATPase with the a3 isoform is the proton pump on premature melanosomes.
Tabata H, Kawamura N, Sun-Wada GH, Wada Y
Cell and tissue research 2008 Jun;332(3):447-60
Cell and tissue research 2008 Jun;332(3):447-60
Rab7 regulates maturation of melanosomal matrix protein gp100/Pmel17/Silv.
Kawakami A, Sakane F, Imai S, Yasuda S, Kai M, Kanoh H, Jin HY, Hirosaki K, Yamashita T, Fisher DE, Jimbow K
The Journal of investigative dermatology 2008 Jan;128(1):143-50
The Journal of investigative dermatology 2008 Jan;128(1):143-50
Cell-specific ATP7A transport sustains copper-dependent tyrosinase activity in melanosomes.
Setty SR, Tenza D, Sviderskaya EV, Bennett DC, Raposo G, Marks MS
Nature 2008 Aug 28;454(7208):1142-6
Nature 2008 Aug 28;454(7208):1142-6
Melanosis in a Holstein heifer.
Oruç E
Journal of veterinary medicine. A, Physiology, pathology, clinical medicine 2007 Nov;54(9):501-3
Journal of veterinary medicine. A, Physiology, pathology, clinical medicine 2007 Nov;54(9):501-3
Cellular defects in Chediak-Higashi syndrome correlate with the molecular genotype and clinical phenotype.
Westbroek W, Adams D, Huizing M, Koshoffer A, Dorward H, Tinloy B, Parkes J, Helip-Wooley A, Kleta R, Tsilou E, Duvernay P, Digre KB, Creel DJ, White JG, Boissy RE, Gahl WA
The Journal of investigative dermatology 2007 Nov;127(11):2674-7
The Journal of investigative dermatology 2007 Nov;127(11):2674-7
Characterization of t(6;11)(p21;q12) in a renal-cell carcinoma of an adult patient.
Pecciarini L, Cangi MG, Lo Cunsolo C, Macri' E, Dal Cin E, Martignoni G, Doglioni C
Genes, chromosomes & cancer 2007 May;46(5):419-26
Genes, chromosomes & cancer 2007 May;46(5):419-26
BLOC-1 is required for cargo-specific sorting from vacuolar early endosomes toward lysosome-related organelles.
Setty SR, Tenza D, Truschel ST, Chou E, Sviderskaya EV, Theos AC, Lamoreux ML, Di Pietro SM, Starcevic M, Bennett DC, Dell'Angelica EC, Raposo G, Marks MS
Molecular biology of the cell 2007 Mar;18(3):768-80
Molecular biology of the cell 2007 Mar;18(3):768-80
Improper trafficking of melanocyte-specific proteins in Hermansky-Pudlak syndrome type-5.
Helip-Wooley A, Westbroek W, Dorward HM, Koshoffer A, Huizing M, Boissy RE, Gahl WA
The Journal of investigative dermatology 2007 Jun;127(6):1471-8
The Journal of investigative dermatology 2007 Jun;127(6):1471-8
Dual loss of ER export and endocytic signals with altered melanosome morphology in the silver mutation of Pmel17.
Theos AC, Berson JF, Theos SC, Herman KE, Harper DC, Tenza D, Sviderskaya EV, Lamoreux ML, Bennett DC, Raposo G, Marks MS
Molecular biology of the cell 2006 Aug;17(8):3598-612
Molecular biology of the cell 2006 Aug;17(8):3598-612
Accumulation of low-avidity anti-melanocortin receptor 1 (anti-MC1R) CD8+ T cells in the lesional skin of a patient with melanoma-related depigmentation.
Wankowicz-Kalinska A, Mailliard RB, Olson K, Graham F, Edington H, Kirkwood JM, Martinek S, Das PK, Storkus WJ
Melanoma research 2006 Apr;16(2):165-74
Melanoma research 2006 Apr;16(2):165-74
Mannose receptor targeting of tumor antigen pmel17 to human dendritic cells directs anti-melanoma T cell responses via multiple HLA molecules.
Ramakrishna V, Treml JF, Vitale L, Connolly JE, O'Neill T, Smith PA, Jones CL, He LZ, Goldstein J, Wallace PK, Keler T, Endres MJ
Journal of immunology (Baltimore, Md. : 1950) 2004 Mar 1;172(5):2845-52
Journal of immunology (Baltimore, Md. : 1950) 2004 Mar 1;172(5):2845-52
Mannose receptor targeting of tumor antigen pmel17 to human dendritic cells directs anti-melanoma T cell responses via multiple HLA molecules.
Ramakrishna V, Treml JF, Vitale L, Connolly JE, O'Neill T, Smith PA, Jones CL, He LZ, Goldstein J, Wallace PK, Keler T, Endres MJ
Journal of immunology (Baltimore, Md. : 1950) 2004 Mar 1;172(5):2845-52
Journal of immunology (Baltimore, Md. : 1950) 2004 Mar 1;172(5):2845-52
HER-2, gp100, and MAGE-1 are expressed in human glioblastoma and recognized by cytotoxic T cells.
Liu G, Ying H, Zeng G, Wheeler CJ, Black KL, Yu JS
Cancer research 2004 Jul 15;64(14):4980-6
Cancer research 2004 Jul 15;64(14):4980-6
A novel splice variant of Pmel17 expressed by human melanocytes and melanoma cells lacking some of the internal repeats.
Nichols SE, Harper DC, Berson JF, Marks MS
The Journal of investigative dermatology 2003 Oct;121(4):821-30
The Journal of investigative dermatology 2003 Oct;121(4):821-30
Proprotein convertase cleavage liberates a fibrillogenic fragment of a resident glycoprotein to initiate melanosome biogenesis.
Berson JF, Theos AC, Harper DC, Tenza D, Raposo G, Marks MS
The Journal of cell biology 2003 May 12;161(3):521-33
The Journal of cell biology 2003 May 12;161(3):521-33
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PMEL Monoclonal Antibody (HMB45) (Product # MA1-34759) and a 32-35kDa band corresponding to PMEL was observed across in SK-MEL-5 in comparison to A-375 and SK-BR-3. Whole cell extracts (30 µg lysate) of SK-MEL-5 (Lane 1), A-375 (Lane 2) and SK-BR-3 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PMEL was performed using 70% confluent log phase SK-MEL-5 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PMEL Monoclonal Antibody (HMB45) (Product # MA1-34759) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic(vesicles) localization. Panel e represents A-375 cells having no expression of PMEL. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
