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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- MA5-37902 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Trypsin 2 Recombinant Rabbit Monoclonal Antibody (3D9O7)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Samples: Mouse pancreas, Rat pancreas Immunogen sequence: MNLLLILTFV AAAVAAPFDD DDKIVGGYIC EENSVPYQVS LNSGYHFCGG SLISEQWVVS AGHCYKSRIQ VRLGEHNIEV LEGNEQFINA AKIIRHPKYN
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 3D9O7
- Vial size
- 100 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Trypsin 2 in extracts of various cell lines. Samples were incubated with Trypsin 2 Monoclonal antibody (Product # MA5-37902) using a dilution of 1:5,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Trypsin 2 in paraffin-embedded rat pancreas. Samples were incubated with Trypsin 2 Monoclonal antibody (Product # MA5-37902) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Trypsin 2 in paraffin-embedded human liver cancer. Samples were incubated with Trypsin 2 Monoclonal antibody (Product # MA5-37902) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
