PA5-34897

antibody from Invitrogen Antibodies

Targeting: IK RED, RER

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34897

Provider

Invitrogen Antibodies

Product name

RED Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa, HepG2.

Concentration

0.77 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using RED Polyclonal Antibody (Product # PA5-34897). Sample (30 µg of whole cell lysate). Lane A: HeLa. Lane B: HepG2 . 7.5% SDS PAGE. RED Polyclonal Antibody (Product # PA5-34897) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RED Polyclonal Antibody detects RED protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RED protein stained by RED Polyclonal Antibody (Product # PA5-34897) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RED Polyclonal Antibody detects IK protein at nucleus on H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. Red Polyclonal Antibody (Product # PA5-34897) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RED Polyclonal Antibody detects IK protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. Red Polyclonal Antibody (Product # PA5-34897) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.