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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-32098 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RED Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (88%), Xenopus laevis (95%), Chicken (97%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.8 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Inactivating Mutations of the IK Gene Weaken Ku80/Ku70-Mediated DNA Repair and Sensitize Endometrial Cancer to Chemotherapy.
Gao C, Jin G, Forbes E, Mangala LS, Wang Y, Rodriguez-Aguayo C, Amero P, Bayraktar E, Yan Y, Lopez-Berestein G, Broaddus RR, Sood AK, Xue F, Zhang W
Cancers 2021 May 20;13(10)
Cancers 2021 May 20;13(10)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RED Polyclonal Antibody (Product # PA5-32098). Sample (30 µg of whole cell lysate). Lane A: A549 . 7.5% SDS PAGE. RED Polyclonal Antibody (Product # PA5-32098) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RED Polyclonal Antibody detects IK protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. Red Polyclonal Antibody (Product # PA5-32098) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RED Polyclonal Antibody detects RED protein at nucleus on mouse brain stem by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain stem. Red Polyclonal Antibody (Product # PA5-32098) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RED Polyclonal Antibody detects RED protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Red Polyclonal Antibody (Product # PA5-32098) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
