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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-32151 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RED Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HepG2.
- Concentration
- 1.01 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RED Polyclonal Antibody (Product # PA5-32151). Sample (30 µg of whole cell lysate). A: 293T . B: A431 . C: HepG2 . 7.5% SDS PAGE. Red Polyclonal Antibody (Product # PA5-32151) diluted at 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RED Polyclonal Antibody detects RED protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RED protein stained by RED Polyclonal Antibody (Product # PA5-32151) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RED Polyclonal Antibody detects RED protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RED protein stained by RED Polyclonal Antibody (Product # PA5-32151) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RED Polyclonal Antibody detects IK protein at cytosol and nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. Red Polyclonal Antibody (Product # PA5-32151) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
