PA5-72114

antibody from Invitrogen Antibodies

Targeting: CASTOR2 GATSL1, GATSL2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-72114

Provider

Invitrogen Antibodies

Product name

GATSL2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GATSL2 in various lysates. Samples were incubated with GATSL2 polyclonal antibody (Product # PA5-72114) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: A549 whole cell lysate; Lane 2: Caco2 whole cell lysate; Lane 3: Hela whole cell lysate; Lane 4: MDA-MB-231 whole cell lysate; Lane 5: HepG2 whole cell lysate; Lane 6: U-2OS whole cell lysate; Lane 7: SK-BR-3 whole cell lysate. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GATSL2 in paraffin-embedded human colon tissue. Samples were incubated with GATSL2 polyclonal antibody (Product # PA5-72114) using a dilution of 1:250 for 15 min at room temperature followed by Leica Bond Polymer Refine Detection. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH 9 0).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GATSL2 in paraffin-embedded human brain tissue. Samples were incubated with GATSL2 polyclonal antibody (Product # PA5-72114) using a dilution of 1:250 for 15 min at room temperature followed by Leica Bond Polymer Refine Detection. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH 9 0).