AF7618

antibody from Novus Biologicals

Targeting: GLIS1 FLJ36155

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF7618

Provider

Novus Biologicals

Product name

Sheep Polyclonal Glis1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human Glis1 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse Glis1 is observed.

Reactivity

Human

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Glis1 by Western Blot. Western blot shows lysates of HEK001 human epidermal keratinocyte cell line, WS-1 human fetal skin fibroblast cell line, U-118-MG human glioblastoma/astrocytoma cell line, and U-87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Glis1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7618) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Glis1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.