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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB100-56119 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56119, RRID:AB_2275590
- Product name
- Rabbit Polyclonal Caspase-9 Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified. This polyclonal antisera recognizes the proform of caspase-9 (~50 kDa), and the large (~35 kDa) and small (~15 kDa) subunits of active/cleaved Caspase-9. Intermediate caspase-9 cleavage forms may also be seen at ~21 kDa.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references PDK4 Deficiency Induces Intrinsic Apoptosis in Response to Starvation in Fibroblasts from Doberman Pinschers with Dilated Cardiomyopathy.
Combretastatin A-4 Analogue: A Dual-Targeting and Tubulin Inhibitor Containing Antitumor Pt(IV) Moiety with a Unique Mode of Action.
Potent anti-cancer effect of 3'-hydroxypterostilbene in human colon xenograft tumors.
IFN-γ combined with targeting of XIAP leads to increased apoptosis-sensitisation of TRAIL resistant pancreatic carcinoma cells.
Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation.
Multiple caspases are activated after traumatic brain injury: evidence for involvement in functional outcome.
Release of caspase-9 from mitochondria during neuronal apoptosis and cerebral ischemia.
Release of caspase-9 from mitochondria during neuronal apoptosis and cerebral ischemia.
Taggart K, Estrada A, Thompson P, Lourenco F, Kirmani S, Suzuki-Hatano S, Pacak CA
BioResearch open access 2017;6(1):182-191
BioResearch open access 2017;6(1):182-191
Combretastatin A-4 Analogue: A Dual-Targeting and Tubulin Inhibitor Containing Antitumor Pt(IV) Moiety with a Unique Mode of Action.
Huang X, Huang R, Gou S, Wang Z, Liao Z, Wang H
Bioconjugate chemistry 2016 Sep 21;27(9):2132-48
Bioconjugate chemistry 2016 Sep 21;27(9):2132-48
Potent anti-cancer effect of 3'-hydroxypterostilbene in human colon xenograft tumors.
Cheng TC, Lai CS, Chung MC, Kalyanam N, Majeed M, Ho CT, Ho YS, Pan MH
PloS one 2014;9(11):e111814
PloS one 2014;9(11):e111814
IFN-γ combined with targeting of XIAP leads to increased apoptosis-sensitisation of TRAIL resistant pancreatic carcinoma cells.
Büneker CK, Yu R, Deedigan L, Mohr A, Zwacka RM
Cancer letters 2012 Mar 28;316(2):168-77
Cancer letters 2012 Mar 28;316(2):168-77
Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation.
Krajewska M, Rosenthal RE, Mikolajczyk J, Stennicke HR, Wiesenthal T, Mai J, Naito M, Salvesen GS, Reed JC, Fiskum G, Krajewski S
Experimental neurology 2004 Oct;189(2):261-79
Experimental neurology 2004 Oct;189(2):261-79
Multiple caspases are activated after traumatic brain injury: evidence for involvement in functional outcome.
Knoblach SM, Nikolaeva M, Huang X, Fan L, Krajewski S, Reed JC, Faden AI
Journal of neurotrauma 2002 Oct;19(10):1155-70
Journal of neurotrauma 2002 Oct;19(10):1155-70
Release of caspase-9 from mitochondria during neuronal apoptosis and cerebral ischemia.
Krajewski S, Krajewska M, Ellerby LM, Welsh K, Xie Z, Deveraux QL, Salvesen GS, Bredesen DE, Rosenthal RE, Fiskum G, Reed JC
Proceedings of the National Academy of Sciences of the United States of America 1999 May 11;96(10):5752-7
Proceedings of the National Academy of Sciences of the United States of America 1999 May 11;96(10):5752-7
Release of caspase-9 from mitochondria during neuronal apoptosis and cerebral ischemia.
Krajewski S, Krajewska M, Ellerby LM, Welsh K, Xie Z, Deveraux QL, Salvesen GS, Bredesen DE, Rosenthal RE, Fiskum G, Reed JC
Proceedings of the National Academy of Sciences of the United States of America 1999 May 11;96(10):5752-7
Proceedings of the National Academy of Sciences of the United States of America 1999 May 11;96(10):5752-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Caspase-9 Antibody [NB100-56119] - Lane view shows a specific band for Caspase 9 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Caspase-9 Antibody [NB100-56119] - Analysis of Caspase-9. Various tissue lysates were prepared from human autospsy material and normalized for total protein. Most tissues contained the ~50 kDa pro-Caspase-9 protein. Cleaved Caspase-9 was identified in brain cortex and peripheral nerve tissue samples. Pro-C9: recombinant human pro-Caspase-9 (full-length) protein. PRO: pro-Caspase-9. L: large subunit of cleaved Caspase-9. S: small subunit of cleaved Caspase-9.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56119] - Mouse brain tissue sections at 1:2000. Hematoxylin-eosin counterstain. A) Brain striatum, B) Brain stem motor neuron.
