Ab093085

antibody from Aladdin Scientific

Targeting: CASP9 APAF-3, ICE-LAP6, MCH6, PPP1R56

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab093085

Provider

Aladdin Scientific

Product name

Caspase-9 Mouse mAb

Antibody type

Monoclonal

Description

Mouse anti Human Caspase-9 Antibody, Monoclonal(1D1-F2-E9), could be used for WB and so on .Application:WB: 1/500-1/1000Protein FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

AA 1-416


Antibody clone number

1D1-F2-E9

Vial size

100_l,10_l,1ml,50_l

Concentration

2,05 mg/ml

Storage

Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Caspase-9 Mouse mAb (Ab093085) - Western Blot All lanes: Caspase-9 Mouse mAb (Ab093085) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/40000 dilution Predicted band size: 46 kDa Observed band size: 27, 43, 45 kDa Exposure time: 30 s