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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-29827 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MLK1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2, Molt-4, Raji, Jurkat, Raji, NCI-H929. Predicted reactivity: Mouse (99%), Rat (97%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.4 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MLK1 Polyclonal Antibody (Product # PA5-29827). Sample (30 µg of whole cell lysate). Lane A: A431 . 7.5% SDS PAGE. MLK1 Polyclonal Antibody (Product # PA5-29827) diluted at 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MAP3K9 antibody detects MAP3K9 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP3K9 antibody MLK1 Polyclonal Antibody (Product # PA5-29827) diluted by 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MLK1/MAP3K9 in paraformaldehyde-fixed A431 cells using a MLK1/MAP3K9 polyclonal antibody (Product # PA5-29827) at a 1:200 dilution.
