14582-1-AP

antibody from Invitrogen Antibodies

Targeting: BRAF BRAF1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14582-1-AP

Provider

Invitrogen Antibodies

Product name

HMG20B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: PVTGYVRFL NERREQIRTR HPDLPFPEIT KMLGAEWSKL QPTEKQRYLD EAEREKQQYM KELRAYQQSE AYKMCTEKIQ EKKIKKEDSS SGLMNTLLNG HKGGDCDGFS TFDVPIFTEE FLDQNKAREA ELRRLRKMNV AFEEQNAVLQ RHTQSMSSAR ERLEQELALE ERRTLALQQQ LQAVRQALTA SFASLPVPGT GETPTLGTLD FYM (73-284 aa encoded by BC002552)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.23 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14582-1-AP (HMG20B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of HMG20B antibody (14582-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HMG20B transfected HeLa cells.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 14582-1-AP (HMG20B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 14582-1-AP (HMG20B antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 14582-1-AP (HMG20B antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-HMG20B (IP:14582-1-AP, 4ug; Detection:14582-1-AP 1:800) with HeLa cells lysate 1200ug.