- PA5-40127 - Invitrogen Antibodies STX17 antibody

Submitted references The CD36 Ligand-Promoted Autophagy Protects Retinal Pigment Epithelial Cells from Oxidative Stress.

Dorion MF, Mulumba M, Kasai S, Itoh K, Lubell WD, Ong H
Oxidative medicine and cellular longevity 2021;2021:6691402

STX17 dynamically regulated by Fis1 induces mitophagy via hierarchical macroautophagic mechanism.

Xian H, Yang Q, Xiao L, Shen HM, Liou YC
Nature communications 2019 May 3;10(1):2059

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of STX17 was performed in DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: STX17 Polyclonal Antibody (Product # PA5-40127) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: STX17 Polyclonal Antibody detects STX17 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: STX17 stained by STX17 Polyclonal Antibody (Product # PA5-40127) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 MPE-001 increased the recruitment of STX17 to autophagosomes in NaIO 3 -treated cells. hTERT RPE-1 cells were pretreated with 1 mu M MPE-001 for 2 h and then exposed to 12.5 mM NaIO 3 for 4 h. (a) Representative images of LC3B-GFP-transfected cells immunostained for STX17 following treatments in the presence of 10 nM bafilomycin A1 (scale bar = 10 mu m). The same gamma correction was applied to all images. (b) Fraction of LC3B puncta that overlap STX17 staining. Manders' colocalization coefficient analysis was carried out across a series of 5 to 6 images of 6 to 18 cells each. Mean +- SD, *** p < 0.001. (c) Immunoblot of STX17 and GAPDH (upper) and relative quantification of STX17/GAPDH (lower). n = 4, mean +- SD, ns: nonsignificant, and ** p < 0.01 vs vehicle.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1 Mitochondrial fission 1 protein (Fis1) and syntaxin 17 (STX17) interact and partially colocalize. a , b HeLa cells were transfected with Flag-tagged vector or Fis1. After 24 h, cells were collected for immunoprecipitation (IP) with anti-Flag beads. Coomassie blue staining was used to visualize bands 1 and 2 ( a ). Results for mass spectrometry analysis of band 1 and 2 are summarized ( b ). c Cells treated as in a were extracted. Anti-Flag immunoprecipitates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotted for STX17 and Flag. Asterisk indicates a non-specific band. d HEK293T cells were co-transfected with Myc-tagged STX17 and Flag-tagged plasmids as indicated. Cells were solubilized for IP with anti-Flag and analyzed with Myc and Flag antibodies respectively. e HeLa cells were transfected with green fluorescent protein (GFP)-tagged vector or STX17 (green) and mCherry-tagged vector or plasmid encoding Fis1 (red) for 24 h. Cells were fixed and stained with anti-Tom20 (cyan). Hoechst, blue. Scale bar, 10 um. f HeLa cells were treated with the indicated small interfering RNA (siRNA) for 24 h before transfecting with GFP-tagged Fis1 (green) or GFP-STX17 (green) for further 24 h. Representative confocal images of live cells are shown. Mitochondrial morphology was visualized using MitoTracker Red (MTR, red). Scale bar, 10 um. White arrowhead indicates cells with decreased MTR. g Quantification of cells with

PA5-40127

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