HPA001204

antibody from Atlas Antibodies

Targeting: STX17 FLJ20651

Western blot (Enhanced validation)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA001204

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA001204, RRID:AB_1080118

Product name

Anti-STX17

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human STX17, Gene description: syntaxin 17, Alternative Gene Names: FLJ20651, Validated applications: WB, IHC, Uniprot ID: P56962, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Deacetylated SNAP47 recruits HOPS to facilitate autophagosome-lysosome fusion independent of STX17

Jian F, Wang S, Tian W, Chen Y, Wang S, Li Y, Ma C, Rong Y
Nature Communications 2025;16(1)

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Exacerbation of atherosclerosis by STX17 knockdown: Unravelling the role of autophagy and inflammation

Cui X, Wang B, Han D, Cheng M, Yuan P, Du P, Hou Y, Su C, Tang J, Zhang J
Journal of Cellular and Molecular Medicine 2024;28(10)

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Multiple Genes Core to ERAD, Macroautophagy and Lysosomal Degradation Pathways Participate in the Proteostasis Response in α1-Antitrypsin Deficiency

Li J, Moretti F, Hidvegi T, Sviben S, Fitzpatrick J, Sundaramoorthi H, Pak S, Silverman G, Knapp B, Filipuzzi I, Alford J, Reece-Hoyes J, Nigsch F, Murphy L, Nyfeler B, Perlmutter D
Cellular and Molecular Gastroenterology and Hepatology 2024;17(6):1007-1024

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C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy

Zhang S, Tong M, Zheng D, Huang H, Li L, Ungermann C, Pan Y, Luo H, Lei M, Tang Z, Fu W, Chen S, Liu X, Zhong Q
Nature Communications 2023;14(1)

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Higher pNRF2, SOCS3, IRF3, and RIG1 Tissue Protein Expression in NASH Patients versus NAFL Patients: pNRF2 Expression Is Concomitantly Associated with Elevated Fasting Glucose Levels

Schwertheim S, Alhardan M, Manka P, Sowa J, Canbay A, Schmidt H, Baba H, Kälsch J
Journal of Personalized Medicine 2023;13(7):1152

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O-GlcNAcylation stabilizes the autophagy-initiating kinase ULK1 by inhibiting chaperone-mediated autophagy upon HPV infection

Shi Y, Yan S, Shao G, Wang J, Jian Y, Liu B, Yuan Y, Qin K, Nai S, Huang X, Wang Y, Chen Z, Chen X, Dong M, Geng Y, Xu Z, Li J
Journal of Biological Chemistry 2022;298(9):102341

---

Persistent coxsackievirus B1 infection triggers extensive changes in the transcriptome of human pancreatic ductal cells

Buchacher T, Honkimaa A, Välikangas T, Lietzén N, Hirvonen M, Laiho J, Sioofy-Khojine A, Eskelinen E, Hyöty H, Elo L, Lahesmaa R
iScience 2022;25(1):103653

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STING controls energy stress-induced autophagy and energy metabolism via STX17

Rong Y, Zhang S, Nandi N, Wu Z, Li L, Liu Y, Wei Y, Zhao Y, Yuan W, Zhou C, Xiao G, Levine B, Yan N, Mou S, Deng L, Tang Z, Liu X, Kramer H, Zhong Q
Journal of Cell Biology 2022;221(7)

---

Acetylation of SCFD1 regulates SNARE complex formation and autophagosome-lysosome fusion

Huang H, Ouyang Q, Mei K, Liu T, Sun Q, Liu W, Liu R
Autophagy 2022;19(1):189-203

---

PACSIN1 is indispensable for amphisome-lysosome fusion during basal autophagy and subsets of selective autophagy

Irazoqui J, Oe Y, Kakuda K, Yoshimura S, Hara N, Hasegawa J, Terawaki S, Kimura Y, Ikenaka K, Suetsugu S, Mochizuki H, Yoshimori T, Nakamura S
PLOS Genetics 2022;18(6):e1010264

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JIB-04, a histone demethylase Jumonji C domain inhibitor, regulates phenotypic switching of vascular smooth muscle cells

He Y, Yi X, Zhang Z, Luo H, Li R, Feng X, Fang Z, Zhu X, Cheng W, Jiang D, Zhao F, Wei X
Clinical Epigenetics 2022;14(1)

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Novel roles of RTN4 and CLIMP-63 in regulating mitochondrial structure, bioenergetics and apoptosis

Carter R, Milani M, Beckett A, Liu S, Prior I, Cohen G, Varadarajan S
Cell Death & Disease 2022;13(5)

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Kansl1 haploinsufficiency impairs autophagosome-lysosome fusion and links autophagic dysfunction with Koolen-de Vries syndrome in mice

Li T, Lu D, Yao C, Li T, Dong H, Li Z, Xu G, Chen J, Zhang H, Yi X, Zhu H, Liu G, Wen K, Zhao H, Gao J, Zhang Y, Han Q, Li T, Zhang W, Zhao J, Li T, Bai Z, Song M, He X, Zhou T, Xia Q, Li A, Pan X
Nature Communications 2022;13(1)

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Muscle Proteomic Profile before and after Enzyme Replacement Therapy in Late-Onset Pompe Disease

Moriggi M, Capitanio D, Torretta E, Barbacini P, Bragato C, Sartori P, Moggio M, Maggi L, Mora M, Gelfi C
International Journal of Molecular Sciences 2021;22(6):2850

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Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes

Kumar S, Javed R, Mudd M, Pallikkuth S, Lidke K, Jain A, Tangavelou K, Gudmundsson S, Ye C, Rusten T, Anonsen J, Lystad A, Claude-Taupin A, Simonsen A, Salemi M, Phinney B, Li J, Guo L, Bradfute S, Timmins G, Eskelinen E, Deretic V
Cell 2021;184(24):5950-5969.e22

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Autophagy deficiency activates rDNA transcription

Xu Y, Wu Y, Wang L, Ren Z, Song L, Zhang H, Qian C, Wang Q, He Z, Wan W
Autophagy 2021;18(6):1338-1349

---

Piperine promotes autophagy flux by P2RX4 activation in SNCA /α-synuclein-induced Parkinson disease model

Li R, Lu Y, Zhang Q, Liu W, Yang R, Jiao J, Liu J, Gao G, Yang H
Autophagy 2021;18(3):559-575

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TNF-induced necroptosis initiates early autophagy events via RIPK3-dependent AMPK activation, but inhibits late autophagy

Wu W, Wang X, Sun Y, Berleth N, Deitersen J, Schlütermann D, Stuhldreier F, Wallot-Hieke N, José Mendiburo M, Cox J, Peter C, Bergmann A, Stork B
Autophagy 2021;17(12):3992-4009

---

Rab32 uses its effector reticulon 3L to trigger autophagic degradation of mitochondria-associated membrane (MAM) proteins

Herrera-Cruz M, Yap M, Tahbaz N, Phillips K, Thomas L, Thomas G, Simmen T
Biology Direct 2021;16(1)

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Acinetobacter baumannii up-regulates LncRNA-GAS5 and promotes the degradation of STX17 by blocking the activation of YY1

An Z, Ding W
Virulence 2021;12(1):1965-1979

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Human enteroviral infection impairs autophagy in clonal INS(832/13) cells and human pancreatic islet cells

Wernersson A, Sarmiento L, Cowan E, Fex M, Cilio C
Diabetologia 2020;63(11):2372-2384

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Mitochondria–lysosome membrane contacts are defective in GDAP1-related Charcot–Marie–Tooth disease

Cantarero L, Juárez-Escoto E, Civera-Tregón A, Rodríguez-Sanz M, Roldán M, Benítez R, Hoenicka J, Palau F
Human Molecular Genetics 2020;29(22):3589-3605

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Mammalian Atg8 proteins and the autophagy factor IRGM control mTOR and TFEB at a regulatory node critical for responses to pathogens

Kumar S, Jain A, Choi S, da Silva G, Allers L, Mudd M, Peters R, Anonsen J, Rusten T, Lazarou M, Deretic V
Nature Cell Biology 2020;22(8):973-985

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Acetylation of STX17 (syntaxin 17) controls autophagosome maturation

Shen Q, Shi Y, Liu J, Su H, Huang J, Zhang Y, Peng C, Zhou T, Sun Q, Wan W, Liu W
Autophagy 2020;17(5):1157-1169

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Lysosomal targeting of autophagosomes by the TECPR domain of TECPR2

Fraiberg M, Tamim-Yecheskel B, Kokabi K, Subic N, Heimer G, Eck F, Nalbach K, Behrends C, Ben-Zeev B, Shatz O, Elazar Z
Autophagy 2020;17(10):3096-3108

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Pancreas-specific SNAP23 depletion prevents pancreatitis by attenuating pathological basolateral exocytosis and formation of trypsin-activating autolysosomes

Dolai S, Takahashi T, Qin T, Liang T, Xie L, Kang F, Miao Y, Xie H, Kang Y, Manuel J, Winter E, Roche P, Cattral M, Gaisano H
Autophagy 2020;17(10):3068-3081

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Syntaxin-17-Dependent Mitochondrial Dynamics Is Essential for Protection against Oxidative-Stress-Induced Apoptosis

Wang B, Xiao X, Huang F, Liu R
Antioxidants 2019;8(11):522

---

Phosphorylation of Syntaxin 17 by TBK1 Controls Autophagy Initiation

Kumar S, Gu Y, Abudu Y, Bruun J, Jain A, Farzam F, Mudd M, Anonsen J, Rusten T, Kasof G, Ktistakis N, Lidke K, Johansen T, Deretic V
Developmental Cell 2019;49(1):130-144.e6

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Mammalian Atg8 proteins regulate lysosome and autolysosome biogenesis through SNARE s

Gu Y, Princely Abudu Y, Kumar S, Bissa B, Choi S, Jia J, Lazarou M, Eskelinen E, Johansen T, Deretic V
The EMBO Journal 2019;38(22)

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A reversible autophagy inhibitor blocks autophagosome–lysosome fusion by preventing Stx17 loading onto autophagosomes

Vats S, Manjithaya R, Subramani S
Molecular Biology of the Cell 2019;30(17):2283-2295

---

Reticulon and CLIMP-63 regulate nanodomain organization of peripheral ER tubules

Schmid S, Gao G, Zhu C, Liu E, Nabi I
PLOS Biology 2019;17(8):e3000355

---

DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B

Tian X, Zheng P, Zhou C, Wang X, Ma H, Ma W, Zhou X, Teng J, Chen J
Autophagy 2019;16(5):797-810

---

SIGMAR1/Sigma-1 receptor ablation impairs autophagosome clearance

Yang H, Shen H, Li J, Guo L
Autophagy 2019;15(9):1539-1557

---

The macrophage-specific V-ATPase subunit ATP6V0D2 restricts inflammasome activation and bacterial infection by facilitating autophagosome-lysosome fusion

Xia Y, Liu N, Xie X, Bi G, Ba H, Li L, Zhang J, Deng X, Yao Y, Tang Z, Yin B, Wang J, Jiang K, Li Z, Choi Y, Gong F, Cheng X, O’Shea J, Chae J, Laurence A, Yang X
Autophagy 2019;15(6):960-975

---

An alternative mitophagy pathway mediated by Rab9 protects the heart against ischemia

Saito T, Nah J, Oka S, Mukai R, Monden Y, Maejima Y, Ikeda Y, Sciarretta S, Liu T, Li H, Baljinnyam E, Fraidenraich D, Fritzky L, Zhai P, Ichinose S, Isobe M, Hsu C, Kundu M, Sadoshima J
Journal of Clinical Investigation 2019;129(2):802-819

---

STX17 dynamically regulated by Fis1 induces mitophagy via hierarchical macroautophagic mechanism

Xian H, Yang Q, Xiao L, Shen H, Liou Y
Nature Communications 2019;10(1)

---

FIP200 Claw Domain Binding to p62 Promotes Autophagosome Formation at Ubiquitin Condensates.

Turco E, Witt M, Abert C, Bock-Bierbaum T, Su MY, Trapannone R, Sztacho M, Danieli A, Shi X, Zaffagnini G, Gamper A, Schuschnig M, Fracchiolla D, Bernklau D, Romanov J, Hartl M, Hurley JH, Daumke O, Martens S
Molecular cell 2019 Apr 18;74(2):330-346.e11

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A novel autophagy inhibitor berbamine blocks SNARE-mediated autophagosome-lysosome fusion through upregulation of BNIP3

Fu R, Deng Q, Zhang H, Hu X, Li Y, Liu Y, Hu J, Luo Q, Zhang Y, Jiang X, Li L, Yang C, Gao N
Cell Death & Disease 2018;9(2)

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Phosphorylation of ULK1 affects autophagosome fusion and links chaperone-mediated autophagy to macroautophagy

Wang C, Wang H, Zhang D, Luo W, Liu R, Xu D, Diao L, Liao L, Liu Z
Nature Communications 2018;9(1)

---

Syntaxin 17 promotes lipid droplet formation by regulating the distribution of acyl-CoA synthetase 3

Kimura H, Arasaki K, Ohsaki Y, Fujimoto T, Ohtomo T, Yamada J, Tagaya M
Journal of Lipid Research 2018;59(5):805-819

---

Oblongifolin C suppresses lysosomal function independently of TFEB nuclear translocation

Wu M, Lao Y, Tan H, Lu G, Ren Y, Zheng Z, Yi J, Fu W, Shen H, Xu H
Acta Pharmacologica Sinica 2018;40(7):929-937

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Autophagosomal YKT6 is required for fusion with lysosomes independently of syntaxin 17

Matsui T, Jiang P, Nakano S, Sakamaki Y, Yamamoto H, Mizushima N
Journal of Cell Biology 2018;217(8):2633-2645

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Mechanism of Stx17 recruitment to autophagosomes via IRGM and mammalian Atg8 proteins

Kumar S, Jain A, Farzam F, Jia J, Gu Y, Choi S, Mudd M, Claude-Taupin A, Wester M, Lidke K, Rusten T, Deretic V
Journal of Cell Biology 2018;217(3):997-1013

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cPKCγ-Modulated Sequential Reactivation of mTOR Inhibited Autophagic Flux in Neurons Exposed to Oxygen Glucose Deprivation/Reperfusion

Hua R, Han S, Zhang N, Dai Q, Liu T, Li J
International Journal of Molecular Sciences 2018;19(5):1380

---

Protocol for Establishing a Multiplex Image-based Autophagy RNAi Screen in Cell Cultures

Jung J, Behrends C
BIO-PROTOCOL 2017;7(17)

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Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator

Jung J, Nayak A, Schaeffer V, Starzetz T, Kirsch A, Müller S, Dikic I, Mittelbronn M, Behrends C
eLife 2017;6

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Transgenic expression of human APOL1 risk variants in podocytes induces kidney disease in mice

Beckerman P, Bi-Karchin J, Park A, Qiu C, Dummer P, Soomro I, Boustany-Kari C, Pullen S, Miner J, Hu C, Rohacs T, Inoue K, Ishibe S, Saleem M, Palmer M, Cuervo A, Kopp J, Susztak K
Nature Medicine 2017;23(4):429-438

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Accumulation of undegraded autophagosomes by expression of dominant-negative STX17 (syntaxin 17) mutants

Uematsu M, Nishimura T, Sakamaki Y, Yamamoto H, Mizushima N
Autophagy 2017;13(8):1452-1464

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The Autophagosomal SNARE Protein Syntaxin 17 Is an Essential Factor for the Hepatitis C Virus Life Cycle

Ren H, Elgner F, Jiang B, Himmelsbach K, Medvedev R, Ploen D, Hildt E, Ou J
Journal of Virology 2016;90(13):5989-6000

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LAMP-2 is required for incorporating syntaxin-17 into autophagosomes and for their fusion with lysosomes

Hubert V, Peschel A, Langer B, Gröger M, Rees A, Kain R
Biology Open 2016;5(10):1516-1529

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Dedicated SNARE s and specialized TRIM cargo receptors mediate secretory autophagy

Kimura T, Jia J, Kumar S, Choi S, Gu Y, Mudd M, Dupont N, Jiang S, Peters R, Farzam F, Jain A, Lidke K, Adams C, Johansen T, Deretic V
The EMBO Journal 2016;36(1):42-60

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Syntaxin-17 delivers PINK1/parkin-dependent mitochondrial vesicles to the endolysosomal system

McLelland G, Lee S, McBride H, Fon E
Journal of Cell Biology 2016;214(3):275-291

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ATG14 promotes membrane tethering and fusion of autophagosomes to endolysosomes

Diao J, Liu R, Rong Y, Zhao M, Zhang J, Lai Y, Zhou Q, Wilz L, Li J, Vivona S, Pfuetzner R, Brunger A, Zhong Q
Nature 2015;520(7548):563-566

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Impairment of autophagosome-lysosome fusion in the buff mutant mice with the VPS33AD251E mutation

Zhen Y, Li W
Autophagy 2015;11(9):1608-1622

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The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17.

Jiang P, Nishimura T, Sakamaki Y, Itakura E, Hatta T, Natsume T, Mizushima N
Molecular biology of the cell 2014 Apr;25(8):1327-37

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-STX17 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Sample type

Human

Protocol

Protocol