A303-075A

antibody from Invitrogen Antibodies

Targeting: ITSN1 ITSN, MGC134948, MGC134949, SH3D1A, SH3P17

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A303-075A

Provider

Invitrogen Antibodies

Product name

Intersectin 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse Intersectin-1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-Intersectin-1 antibody (Product # A303-075A; lot A303-075A-2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Intersectin 1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Intersectin 1 antibody A303-075A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. Intersectin 1 was also immunoprecipitated by rabbit anti-Intersectin 1 antibody BL11132, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Intersectin-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-Intersectin-1 antibody (Product # A303-075A; lot A303-075A-2) used for IP at 6 µg per reaction. Intersectin-1 was also immunoprecipitated by a previous lot of this antibody (lot A303-075A-1). For blotting immunoprecipitated Intersectin-1, Product # A303-075A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.