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LearnNBP1-71832
antibody from Novus Biologicals
Targeting: ITSN1
ITSN, MGC134948, MGC134949, SH3D1A, SH3P17
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [2]
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- Product number
- NBP1-71832 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-71832, RRID:AB_11017127
- Product name
- Rabbit Polyclonal Intersectin 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Intersectin 1 Antibody [NBP1-71832] - Detection of human and mouse Intersectin-1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-Intersectin-1 antibody NBP1-71832 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Previous
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Intersectin 1 Antibody [NBP1-71832] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Intersectin 1 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Intersectin 1 was also immunoprecipitated by rabbit anti-Intersectin 1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Intersectin 1 Antibody [NBP1-71832] - Detection of human Intersectin-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-Intersectin-1 antibody NBP1-71832 (lot NBP1-71832-2) used for IP at 6 ug per reaction. Intersectin-1 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-71832-1). For blotting immunoprecipitated Intersectin-1, NBP1-71832 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
