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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- NBP2-17260 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal MEF2C Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MEF2C Antibody [NBP2-17260] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:10000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MEF2C Antibody [NBP2-17260] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with MEF2C antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MEF2C Antibody [NBP2-17260] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with MEF2C antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MEF2C Antibody [NBP2-17260] - K562 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of MEF2C protein by 4 ug MEF2C antibody 5 % SDS-PAGE The immunoprecipitated MEF2C protein was detected by MEF2C antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MEF2C Antibody [NBP2-17260] - Mouse fore brain. MEF2C antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MEF2C Antibody [NBP2-17260] - Saos2 xenograft. MEF2C antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: MEF2C Antibody [NBP2-17260] - Mouse cerebral cortex. Green: MEF2C stained by MEF2C antibody diluted at 1:250. Blue: Fluoroshield with DAPI.
