Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AF6786 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal MEF2C Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human MEF2C in direct ELISAs and Western blots.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human MEF2C by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear (Nuc) extracts. PVDF Membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse MEF2C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6786) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for MEF2C at approximately 52-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- MEF2C in Human Brain. MEF2C was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human/ Mouse MEF2C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6786) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- MEF2C in Mouse Brain. MEF2C was detected in perfusion fixed frozen sections of mouse brain (cortex) using Sheep Anti-Human/Mouse MEF2C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6786) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal nuclei. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.