GTX81916

antibody from GeneTex

Targeting: MEF2C

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX81916

Provider

GeneTex

Proper citation

GeneTex Cat#GTX81916, RRID:AB_11170996

Product name

MEF2C antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

MEF2C Antibody (pS387) (GTX81916) western blot analysis in HL-60,K562 cell line lysates (35μg/lane).This demonstrates the MEF2C antibody detected the MEF2C protein (arrow).

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Fluorescent confocal image of Hela cell stained with MEF2C Antibody (S387)(GTX81916).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEF2C primary antibody (1:25, 1 h at 37¢J). For secondary antibody, Alexa Fluor? 488 conjμgated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37¢J).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjμgated Phalloidin (7units/ml, 1 h at 37¢J). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min).MEF2C immunoreactivity is localized to vesicles and Nucleus significantly.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human brain tissue reacted with MEF2C Antibody (S387) (GTX81916), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.