PAB0527

antibody from Abnova Corporation

Targeting: MEF2C

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB0527

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB0527, RRID:AB_982681

Product name

MEF2C (phospho T20) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of MEF2C.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Membrane hyperpolarization triggers myogenin and myocyte enhancer factor-2 expression during human myoblast differentiation.

Konig S, Hinard V, Arnaudeau S, Holzer N, Potter G, Bader CR, Bernheim L
The Journal of biological chemistry 2004 Jul 2;279(27):28187-96

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TEF-1 and MEF2 transcription factors interact to regulate muscle-specific promoters.

Maeda T, Gupta MP, Stewart AF
Biochemical and biophysical research communications 2002 Jun 21;294(4):791-7

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Mammalian vestigial-like 2, a cofactor of TEF-1 and MEF2 transcription factors that promotes skeletal muscle differentiation.

Maeda T, Chapman DL, Stewart AF
The Journal of biological chemistry 2002 Dec 13;277(50):48889-98

Other assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Dot blot analysis of MEF2C (phospho T20) polyclonal antibody (Cat # PAB0527) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5 ug/mL. P-Pab : phospho-antibody; P-Peptide : phospho-peptide; NP-Peptide : non-phospho-peptide.

Validation comment

Dot Blot (Peptide)