HPA005533

MEF2C antibody from Atlas Antibodies

Eligible for validation within the Antibodypedia Validation Initiative

Western blot

Enhanced validation

Immunocytochemistry

Supportive data in Antibodypedia

Immunohistochemistry

Enhanced validation

Provider product page for HPA005533

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Antibody Data

Product number

HPA005533

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA005533, RRID:AB_888646

Product name

Anti-MEF2C

Provider product page

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

PPNFEMPVSIPVSSHNSLVYSNPVSSLGNPNLLPL
AHPSLQRNSMSPGVTHRPPSAGNTGGLMGGDLTSG
AGTSAGNGYGNPRNSPGLLVSPGNLNKNMQAKSPP
PMNLGMNNRKPDLRVLIPPGSKNTMPSVNQRINN


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Mef2c Regulates Transcription of the Extracellular Matrix Protein Cartilage Link Protein 1 in the Developing Murine Heart
Lockhart M, Wirrig E, Phelps A, Ghatnekar A, Barth J, Norris R, Wessels A, Dettman R
PLoS ONE 2013 February;8(2)

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Evolutionary conservation of Nkx2.5 autoregulation in the second heart field
Clark C, Zhang B, Lee B, Evans S, Lassar A, Lee K
Developmental Biology 2013 February;374(1):198-209

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Differential expression of cartilage and bone-related proteins in pediatric and adult diseased aortic valves
Wirrig E, Hinton R, Yutzey K
Journal of Molecular and Cellular Cardiology 2011 March;50(3):561-569

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MicroRNA-21 dysregulates the expression of MEF2C in neurons in monkey and human SIV/HIV neurological disease
Yelamanchili S, Chaudhuri A, Chen L, Xiong H, Fox H
Cell Death and Disease 2010 September;1(9)

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and MEF2C over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419349).

Sample type

HUMAN

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & vesicles.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows low expression as expected.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using Anti-MEF2C antibody. Corresponding MEF2C RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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