Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Other assay [1]
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Validation data
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- Product number
- GTX128961 - Provider product page
- Provider
- GeneTex
- Product name
- Histone H2AK5ac (acetyl Lys5) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Histone H2AK5ac (acetyl Lys5) antibody detects Histone H2AK5ac (acetyl Lys5) protein by Western blot analysis.A. 30 μg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr)B. 30 μg HeLa whole cell lysate/extract (0.4 £gM Trichostatin A treatment for 18 hr)15 % SDS-PAGEHistone H2AK5ac (acetyl Lys5) antibody (GTX128961) dilution: 1:10000
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Histone H2AK5ac (acetyl Lys5) antibody detects Histone H2AK5ac (acetyl Lys5) protein at nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 0.4 £gM TSA for 18 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H2AK5ac (acetyl Lys5) protein stained by Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- Histone H2AK5ac (acetyl Lys5) antibody immunoprecipitates Histone H2AK5ac (acetyl Lys5) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of Histone H2AK5ac (acetyl Lys5) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B : Control with 2.5 £gg of pre-immune rabbit IgG C : Immunoprecipitation of Histone H2AK5ac (acetyl Lys5) protein by 2.5 £gg of Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) 12% SDS-PAGE The immunoprecipitated Histone H2AK5ac (acetyl Lys5) protein was detected by Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) diluted at 1 : 10000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- Dotblot analysis of anti-Histone H2AK5ac (acetyl Lys5) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) 1:5000 dilution.A: Peptide samples of H2A.2K5 unB: Peptide samples of H2A.2K5acC: Peptide samples of H2BK20 unD: Peptide samples of H2BK20acE: Peptide samples of H2BK5 unF: Peptide samples of H2BK5ac
- Validation comment
- Dot