NB600-534
antibody from Novus Biologicals
Targeting: SIGLEC1
CD169, dJ1009E24.1, FLJ00051, FLJ00055, FLJ00073, FLJ32150, sialoadhesin, SIGLEC-1, SN
Antibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
Reference
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- Product number
- NB600-534 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-534, RRID:AB_2189038
- Product name
- Mouse Monoclonal Siglec-1/CD169 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Porcine
- Host
- Mouse
- Antigen sequence
Within domains 1-4.
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Proteomic Profiling Unravels a Key Role of Specific Macrophage Subtypes in Sporadic Inclusion Body Myositis.
Suboptimal Level of Bone-Forming Cells in Advanced Cirrhosis are Associated with Hepatic Osteodystrophy.
Doxorubicin triggers splenic contraction and irreversible dysregulation of COX and LOX that alters the inflammation-resolution program in the myocardium.
A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV.
Musculoskeletal Disease in MDA5-Related Type I Interferonopathy: A Mendelian Mimic of Jaccoud's Arthropathy.
Interferon-Inducible CD169/Siglec1 Attenuates Anti-HIV-1 Effects of Alpha Interferon.
Inhibition of the Unfolded Protein Response Mechanism Prevents Cardiac Fibrosis.
Toll-like receptor 7 involves the injury in acute kidney ischemia/reperfusion of STZ-induced diabetic rats.
Relationships of CD163 and CD169 positive cell numbers in the endometrium and fetal placenta with type 2 PRRSV RNA concentration in fetal thymus.
Three-dimensional reconstruction of serial sections for analysis of the microvasculature of the white pulp and the marginal zone in the human spleen.
CD169-mediated trafficking of HIV to plasma membrane invaginations in dendritic cells attenuates efficacy of anti-gp120 broadly neutralizing antibodies.
Interferon-inducible mechanism of dendritic cell-mediated HIV-1 dissemination is dependent on Siglec-1/CD169.
DEC-205/CD205+ dendritic cells are abundant in the white pulp of the human spleen, including the border region between the red and white pulp.
Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity.
Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations.
Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations.
The species-specific structure of microanatomical compartments in the human spleen: strongly sialoadhesin-positive macrophages occur in the perifollicular zone, but not in the marginal zone.
The species-specific structure of microanatomical compartments in the human spleen: strongly sialoadhesin-positive macrophages occur in the perifollicular zone, but not in the marginal zone.
Roos A, Preusse C, Hathazi D, Goebel HH, Stenzel W
Frontiers in immunology 2019;10:1040
Frontiers in immunology 2019;10:1040
Suboptimal Level of Bone-Forming Cells in Advanced Cirrhosis are Associated with Hepatic Osteodystrophy.
Bihari C, Lal D, Thakur M, Sukriti S, Mathur D, Patil AG, Anand L, Kumar G, Sharma S, Thapar S, Rajbongshi A, Rastogi A, Kumar A, Sarin SK
Hepatology communications 2018 Sep;2(9):1095-1110
Hepatology communications 2018 Sep;2(9):1095-1110
Doxorubicin triggers splenic contraction and irreversible dysregulation of COX and LOX that alters the inflammation-resolution program in the myocardium.
Jadapalli JK, Wright GW, Kain V, Sherwani MA, Sonkar R, Yusuf N, Halade GV
American journal of physiology. Heart and circulatory physiology 2018 Nov 1;315(5):H1091-H1100
American journal of physiology. Heart and circulatory physiology 2018 Nov 1;315(5):H1091-H1100
A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV.
OhAinle M, Helms L, Vermeire J, Roesch F, Humes D, Basom R, Delrow JJ, Overbaugh J, Emerman M
eLife 2018 Dec 6;7
eLife 2018 Dec 6;7
Musculoskeletal Disease in MDA5-Related Type I Interferonopathy: A Mendelian Mimic of Jaccoud's Arthropathy.
de Carvalho LM, Ngoumou G, Park JW, Ehmke N, Deigendesch N, Kitabayashi N, Melki I, Souza FFL, Tzschach A, Nogueira-Barbosa MH, Ferriani V, Louzada-Junior P, Marques W Jr, Lourenço CM, Horn D, Kallinich T, Stenzel W, Hur S, Rice GI, Crow YJ
Arthritis & rheumatology (Hoboken, N.J.) 2017 Oct;69(10):2081-2091
Arthritis & rheumatology (Hoboken, N.J.) 2017 Oct;69(10):2081-2091
Interferon-Inducible CD169/Siglec1 Attenuates Anti-HIV-1 Effects of Alpha Interferon.
Akiyama H, Ramirez NP, Gibson G, Kline C, Watkins S, Ambrose Z, Gummuluru S
Journal of virology 2017 Nov 1;91(21)
Journal of virology 2017 Nov 1;91(21)
Inhibition of the Unfolded Protein Response Mechanism Prevents Cardiac Fibrosis.
Groenendyk J, Lee D, Jung J, Dyck JR, Lopaschuk GD, Agellon LB, Michalak M
PloS one 2016;11(7):e0159682
PloS one 2016;11(7):e0159682
Toll-like receptor 7 involves the injury in acute kidney ischemia/reperfusion of STZ-induced diabetic rats.
Yayi H, Yeda X, Huaxin W, Yang W, Qian S, Zhongyuan X
Acta cirurgica brasileira 2016 Jul;31(7):448-55
Acta cirurgica brasileira 2016 Jul;31(7):448-55
Relationships of CD163 and CD169 positive cell numbers in the endometrium and fetal placenta with type 2 PRRSV RNA concentration in fetal thymus.
Novakovic P, Harding JC, Ladinig A, Al-Dissi AN, MacPhee DJ, Detmer SE
Veterinary research 2016 Aug 5;47(1):76
Veterinary research 2016 Aug 5;47(1):76
Three-dimensional reconstruction of serial sections for analysis of the microvasculature of the white pulp and the marginal zone in the human spleen.
Kusumi S, Koga D, Kanda T, Ushiki T
Biomedical research (Tokyo, Japan) 2015;36(3):195-203
Biomedical research (Tokyo, Japan) 2015;36(3):195-203
CD169-mediated trafficking of HIV to plasma membrane invaginations in dendritic cells attenuates efficacy of anti-gp120 broadly neutralizing antibodies.
Akiyama H, Ramirez NG, Gudheti MV, Gummuluru S
PLoS pathogens 2015 Mar;11(3):e1004751
PLoS pathogens 2015 Mar;11(3):e1004751
Interferon-inducible mechanism of dendritic cell-mediated HIV-1 dissemination is dependent on Siglec-1/CD169.
Puryear WB, Akiyama H, Geer SD, Ramirez NP, Yu X, Reinhard BM, Gummuluru S
PLoS pathogens 2013;9(4):e1003291
PLoS pathogens 2013;9(4):e1003291
DEC-205/CD205+ dendritic cells are abundant in the white pulp of the human spleen, including the border region between the red and white pulp.
Pack M, Trumpfheller C, Thomas D, Park CG, Granelli-Piperno A, Münz C, Steinman RM
Immunology 2008 Mar;123(3):438-46
Immunology 2008 Mar;123(3):438-46
Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity.
Rempel H, Calosing C, Sun B, Pulliam L
PloS one 2008 Apr 16;3(4):e1967
PloS one 2008 Apr 16;3(4):e1967
Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations.
Hartnell A, Steel J, Turley H, Jones M, Jackson DG, Crocker PR
Blood 2001 Jan 1;97(1):288-96
Blood 2001 Jan 1;97(1):288-96
Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations.
Hartnell A, Steel J, Turley H, Jones M, Jackson DG, Crocker PR
Blood 2001 Jan 1;97(1):288-96
Blood 2001 Jan 1;97(1):288-96
The species-specific structure of microanatomical compartments in the human spleen: strongly sialoadhesin-positive macrophages occur in the perifollicular zone, but not in the marginal zone.
Steiniger B, Barth P, Herbst B, Hartnell A, Crocker PR
Immunology 1997 Oct;92(2):307-16
Immunology 1997 Oct;92(2):307-16
The species-specific structure of microanatomical compartments in the human spleen: strongly sialoadhesin-positive macrophages occur in the perifollicular zone, but not in the marginal zone.
Steiniger B, Barth P, Herbst B, Hartnell A, Crocker PR
Immunology 1997 Oct;92(2):307-16
Immunology 1997 Oct;92(2):307-16
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Staining of Sialoadhesin in human spleen using DAB with hematoxylin counterstain.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Adult mouse small intestine. NB600-534 Siglec-1 was used at 1:500 and labeled with Alexa Fluor 568 conjugated secondary antibody (Red). DAPI shown as blue. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Human CD14+ PBMC differentiated to M1 macrophages with rhGM-CSF were stained with Mouse Anti-Siglec-1/CD169 Monoclonal Antibody (NB600-534, filled histogram), or Mouse IgG1 isotype control (MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).