AF5197
antibody from R&D Systems
Targeting: SIGLEC1
CD169, dJ1009E24.1, FLJ00051, FLJ00055, FLJ00073, FLJ32150, sialoadhesin, SIGLEC-1, SN
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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Validation data
Reference
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- Product number
- AF5197 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Siglec-1/CD169 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Siglec-1/CD169 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Siglec-1 is observed and less than 1% cross-reactivity with recombinant human Siglec-2 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q9BZZ2
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Siglec-1 initiates formation of the virus-containing compartment and enhances macrophage-to-T cell transmission of HIV-1.
Distinct patterns and prognostic values of tumor-infiltrating macrophages in hepatocellular carcinoma and gastric cancer.
Hammonds JE, Beeman N, Ding L, Takushi S, Francis AC, Wang JJ, Melikyan GB, Spearman P
PLoS pathogens 2017 Jan;13(1):e1006181
PLoS pathogens 2017 Jan;13(1):e1006181
Distinct patterns and prognostic values of tumor-infiltrating macrophages in hepatocellular carcinoma and gastric cancer.
Li JQ, Yu XJ, Wang YC, Huang LY, Liu CQ, Zheng L, Fang YJ, Xu J
Journal of translational medicine 2017 Feb 15;15(1):37
Journal of translational medicine 2017 Feb 15;15(1):37
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Siglec-1 by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and mouse lymph node tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Siglec-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5197) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Siglec-1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Adhesion Mediated by Siglec-1/CD169 and Neutralization by Human Siglec-1/CD169 Antibody. Recombinant Human Siglec-1/CD169 Fc Chimera (Catalog # 5197-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Siglec-1/CD169 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human Siglec-1/CD169 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5197). The ND50 is typically 1.5-7.5 µg/mL.