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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 657702 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#657702, RRID:AB_2562646
- Product name
- Purified anti-WASH1
- Antibody type
- Monoclonal
- Antigen
- GST fusion to full length human WASH-1
- Description
- Wiskott-Aldrich Syndrome Protein and SCAR Homolog (WASH) is a group of nucleation promoting factors that belongs to the WASP family. WASH-1 is evolutionarily conserved and is widely expressed in human tissues. It is located on endosome membranes and plays critical roles in the recruitment and activation of Arp2/3 in order to initiate actin polymerization. Mutations in the Wiskott-Aldrich Syndrome gene is the cause of Wiskott-Aldrich syndrome and X-linked neutropenia.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- P1B1-B1
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Hela cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-WASH-1 antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated
