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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- HPA002689 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA002689, RRID:AB_1078803
- Product name
- Anti-WASHC1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human WASHC1, Gene description: WASH complex subunit 1, Alternative Gene Names: FAM39E, FLJ00038, WASH1, Validated applications: ICC, IHC, WB, Uniprot ID: A8K0Z3, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references The Parkinson's disease related mutant VPS35 (D620N) amplifies the LRRK2 response to endolysosomal stress
Actin cables and comet tails organize mitochondrial networks in mitosis
Genome-scale CRISPR screening for potential targets of ginsenoside compound K
Loss of strumpellin in the melanocytic lineage impairs theWASHComplex but does not affect coat colour
WASH drives early recycling from macropinosomes and phagosomes to maintain surface phagocytic receptors
McCarron K, Elcocks H, Mortiboys H, Urbé S, Clague M
Biochemical Journal 2024;481(4):265-278
Biochemical Journal 2024;481(4):265-278
Actin cables and comet tails organize mitochondrial networks in mitosis
Moore A, Coscia S, Simpson C, Ortega F, Wait E, Heddleston J, Nirschl J, Obara C, Guedes-Dias P, Boecker C, Chew T, Theriot J, Lippincott-Schwartz J, Holzbaur E
Nature 2021;591(7851):659-664
Nature 2021;591(7851):659-664
Genome-scale CRISPR screening for potential targets of ginsenoside compound K
Yang Y, Liu X, Li S, Chen Y, Zhao Y, Wei Y, Qiu Y, Liu Y, Zhou Z, Han J, Wu G, Ding Q
Cell Death & Disease 2020;11(1)
Cell Death & Disease 2020;11(1)
Loss of strumpellin in the melanocytic lineage impairs theWASHComplex but does not affect coat colour
Tyrrell B, Woodham E, Spence H, Strathdee D, Insall R, Machesky L
Pigment Cell & Melanoma Research 2016;29(5):559-571
Pigment Cell & Melanoma Research 2016;29(5):559-571
WASH drives early recycling from macropinosomes and phagosomes to maintain surface phagocytic receptors
Buckley C, Gopaldass N, Bosmani C, Johnston S, Soldati T, Insall R, King J
Proceedings of the National Academy of Sciences 2016;113(40)
Proceedings of the National Academy of Sciences 2016;113(40)
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-WASHC1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
- Sample type
- Human
