NBP2-23490

antibody from Novus Biologicals

Targeting: SQSTM1 A170, OSIL, p60, p62, p62B, PDB3

Provider product page for NBP2-23490

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [5]
Comments [0]
Validations
Western blot [4]
Immunohistochemistry [3]
Flow cytometry [1]

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Product number: NBP2-23490 - Provider product page
Provider: Novus Biologicals
Product name: Mouse Monoclonal p62/SQSTM1 Antibody
Antibody type: Monoclonal
Description: Ammonium sulfate precipitation.
Reactivity: Human, Simian
Host: Mouse
Isotype: IgG
Vial size: 0.1 ml
Concentration: 1.0 mg/ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

SQSTM1 protein structure - NBP2-23490 shown in red.

Submitted references Age-Related Intraneuronal Aggregation of Amyloid-β in Endosomes, Mitochondria, Autophagosomes, and Lysosomes.

Brewer GJ, Herrera RA, Philipp S, Sosna J, Reyes-Ruiz JM, Glabe CG
Journal of Alzheimer's disease : JAD 2020;73(1):229-246

Generation and Characterization of Novel Monoclonal Antibodies Targeting p62/sequestosome-1 Across Human Neurodegenerative Diseases.

Trejo-Lopez JA, Sorrentino ZA, Riffe CJ, Prokop S, Dickson DW, Yachnis AT, Giasson BI
Journal of neuropathology and experimental neurology 2020 Apr 1;79(4):407-418

Impaired autophagic flux and its related inflammation in patients with adult-onset Still's disease.

Hsieh CW, Chang CY, Chen YM, Chen HH, Hung WT, Gung NR, Wey SJ, Chen DY
Oncotarget 2018 Jan 2;9(1):110-121

Association between autophagy and inflammation in patients with rheumatoid arthritis receiving biologic therapy.

Chen YM, Chang CY, Chen HH, Hsieh CW, Tang KT, Yang MC, Lan JL, Chen DY
Arthritis research & therapy 2018 Dec 5;20(1):268

Inhibition of ERα/ERK/P62 cascades induces "autophagic switch" in the estrogen receptor-positive breast cancer cells exposed to gemcitabine.

Shen P, Chen M, He M, Chen L, Song Y, Xiao P, Wan X, Dai F, Pan T, Wang Q
Oncotarget 2016 Jul 26;7(30):48501-48516

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: p62/SQSTM1 Antibody (5H7E2) [NBP2-23490] - Analysis using p62/SQSTM1 mAb against human SQSTM1 recombinant protein. (Expected MW is 39.1 kDa)

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: p62/SQSTM1 Antibody (5H7E2) [NBP2-23490] - Analysis using p62/SQSTM1 mAb in (1) HEK293 and (2) SQSTM1 hIgGFc transfected HEK293 cell lysate.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: p62/SQSTM1 Antibody (5H7E2) [NBP2-23490] - Detection of p62 protein in lysates of Hela, Jurkat, THP-1, COS7, and SK-BR-3 cell lines using p62/SQSTM1 antibody clone 5H7E2. The antibody detected a specific band of p62 at ~62kDa position.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: p62/SQSTM1 Antibody (5H7E2) [NBP2-23490] - Analysis of p62 protein in lysates of HEK293, A549, MCF-7, and HepG2 cell lines using p62/SQSTM1 antibody clone 5H7E2. The antibody detected a specific band at ~62kDa position.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: p62/SQSTM1 Antibody (5H7E2) [NBP2-23490] - Analysis of paraffin-embedded human cervical cancer tissues using p62/SQSTM1 mouse mAb with DAB staining.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: p62/SQSTM1 Antibody (5H7E2) [NBP2-23490] - Analysis of paraffin-embedded bladder cancer tissues using SQSTM1 mouse mAb with DAB staining.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: p62/SQSTM1 Antibody (5H7E2) [NBP2-23490] - Antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: p62/SQSTM1 Antibody (5H7E2) [NBP2-23490] - Analysis of HEK293 cells using SQSTM1 mouse mAb (green) and negative control (red).