- GTX100685 - GeneTex SQSTM1 antibody

Submitted references Human non‑small cell lung cancer cells can be sensitized to camptothecin by modulating autophagy.

Chiu YH, Hsu SH, Hsu HW, Huang KC, Liu W, Wu CY, Huang WP, Chen JY, Chen BH, Chiu CC
International journal of oncology 2018 Nov;53(5):1967-1979

Effects and mechanisms of betulinic acid on improving EGFR TKI-resistance of lung cancer cells.

Ko JL, Lin CH, Chen HC, Hung WH, Chien PJ, Chang HY, Wang BY
Environmental toxicology 2018 Nov;33(11):1153-1159

Induction of N-Ras degradation by flunarizine-mediated autophagy.

Zheng ZY, Li J, Li F, Zhu Y, Cui K, Wong ST, Chang EC, Liao YH
Scientific reports 2018 Nov 16;8(1):16932

Dual Inhibition of PIK3C3 and FGFR as a New Therapeutic Approach to Treat Bladder Cancer.

Chen CH, Changou CA, Hsieh TH, Lee YC, Chu CY, Hsu KC, Wang HC, Lin YC, Lo YN, Liu YR, Liou JP, Yen Y
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Mar 1;24(5):1176-1189

PHLPP2 stabilization by p27 mediates its inhibition of bladder cancer invasion by promoting autophagic degradation of MMP2 protein.

Peng M, Wang J, Zhang D, Jin H, Li J, Wu XR, Huang C
Oncogene 2018 Jun 21;

Improving nuclear envelope dynamics by EBV BFRF1 facilitates intranuclear component clearance through autophagy.

Liu GT, Kung HN, Chen CK, Huang C, Wang YL, Yu CP, Lee CP
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Jul;32(7):3968-3983

Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.

Liu YH, Weng YP, Tsai HY, Chen CJ, Lee DY, Hsieh CL, Wu YC, Lin JY
Phytomedicine : international journal of phytotherapy and phytopharmacology 2018 Jul 15;46:184-192

Resveratrol provides neuroprotective effects through modulation of mitochondrial dynamics and ERK1/2 regulated autophagy.

Lin KL, Lin KJ, Wang PW, Chuang JH, Lin HY, Chen SD, Chuang YC, Huang ST, Tiao MM, Chen JB, Huang PH, Liou CW, Lin TK
Free radical research 2018 Dec;52(11-12):1371-1386

Hispolon suppresses metastasis via autophagic degradation of cathepsin S in cervical cancer cells.

Hsin MC, Hsieh YH, Wang PH, Ko JL, Hsin IL, Yang SF
Cell death & disease 2017 Oct 5;8(10):e3089

The Adaptor Protein p62 Mediates Nuclear Factor κB Activation in Response to Inflammation and Facilitates the Formation of Prolabor Mediators in Human Myometrium.

Lappas M
Reproductive sciences (Thousand Oaks, Calif.) 2017 May;24(5):762-772

TRIM14 is a Putative Tumor Suppressor and Regulator of Innate Immune Response in Non-Small Cell Lung Cancer.

Hai J, Zhu CQ, Wang T, Organ SL, Shepherd FA, Tsao MS
Scientific reports 2017 Jan 6;7:39692

Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells.

Liu YH, Weng YP, Lin HY, Tang SW, Chen CJ, Liang CJ, Ku CY, Lin JY
Scientific reports 2017 Jan 30;7:41437

Atg5-mediated autophagy deficiency in proximal tubules promotes cell cycle G2/M arrest and renal fibrosis.

Li H, Peng X, Wang Y, Cao S, Xiong L, Fan J, Wang Y, Zhuang S, Yu X, Mao H
Autophagy 2016 Sep;12(9):1472-86

Mycotoxin Patulin Suppresses Innate Immune Responses by Mitochondrial Dysfunction and p62/Sequestosome-1-dependent Mitophagy.

Tsai WT, Lo YC, Wu MS, Li CY, Kuo YP, Lai YH, Tsai Y, Chen KC, Chuang TH, Yao CH, Lee JC, Hsu LC, Hsu JT, Yu GY
The Journal of biological chemistry 2016 Sep 9;291(37):19299-311

Cellular degradation activity is maintained during aging in long-living queen bees.

Hsu CY, Qiu JT, Chan YP
Biogerontology 2016 Nov;17(5-6):829-840

1-(2-Hydroxy-5-methylphenyl)-3-phenyl-1,3-propanedione Induces G1 Cell Cycle Arrest and Autophagy in HeLa Cervical Cancer Cells.

Tsai JH, Hsu LS, Huang HC, Lin CL, Pan MH, Hong HM, Chen WJ
International journal of molecular sciences 2016 Aug 5;17(8)

Immunomodulatory Protein from Ganoderma microsporum Induces Pro-Death Autophagy through Akt-mTOR-p70S6K Pathway Inhibition in Multidrug Resistant Lung Cancer Cells.

Chiu LY, Hu ME, Yang TY, Hsin IL, Ko JL, Tsai KJ, Sheu GT
PloS one 2015;10(5):e0125774

S-Adenosyl-L-methionine-competitive inhibitors of the histone methyltransferase EZH2 induce autophagy and enhance drug sensitivity in cancer cells.

Liu TP, Lo HL, Wei LS, Hsiao HH, Yang PM
Anti-cancer drugs 2015 Feb;26(2):139-47

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Li JR, Cheng CL, Yang WJ, Yang CR, Ou YC, Wu MJ, Ko JL
Anticancer research 2014 Jun;34(6):2973-83

Inhibition of G9a induces DUSP4-dependent autophagic cell death in head and neck squamous cell carcinoma.

Li KC, Hua KT, Lin YS, Su CY, Ko JY, Hsiao M, Kuo ML, Tan CT
Molecular cancer 2014 Jul 15;13:172

Metabolism and mis-metabolism of the neuropathological signature protein TDP-43.

Huang CC, Bose JK, Majumder P, Lee KH, Huang JT, Huang JK, Shen CK
Journal of cell science 2014 Jul 15;127(Pt 14):3024-38

Changes in cellular degradation activity in young and old worker honeybees (Apis mellifera).

Hsu CY, Chuang YL, Chan YP
Experimental gerontology 2014 Feb;50:128-36

Presenilin-1 regulates the expression of p62 to govern p62-dependent tau degradation.

Tung YT, Wang BJ, Hsu WM, Hu MK, Her GM, Huang WP, Liao YF
Molecular neurobiology 2014 Feb;49(1):10-27

SIRT3 deacetylates FOXO3 to protect mitochondria against oxidative damage.

Tseng AH, Shieh SS, Wang DL
Free radical biology & medicine 2013 Oct;63:222-34

Dual inhibitor of phosphoinositide 3-kinase/mammalian target of rapamycin NVP-BEZ235 effectively inhibits cisplatin-resistant urothelial cancer cell growth through autophagic flux.

Li JR, Cheng CL, Yang CR, Ou YC, Wu MJ, Ko JL
Toxicology letters 2013 Jul 18;220(3):267-76

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HepG2 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KD Reagent Type: siRNA
Downregulation: >75%
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
KD Reagent Prod page: KD Reagent Prod page
Antibody Lot Number: 41052

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A: A549 10% SDS PAGE GTX100685 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot analysis.A. 30 ?g NIH-3T3 whole cell lysate/extract B. 30 ?g JC whole cell lysate/extract C. 30 ?g BCL-1 whole cell lysate/extract 12% SDS-PAGESQSTM1 antibody [N3C1], Internal (GTX100685) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by Western blot analysis.A. 20 µg Huh7 whole cell lysate/extract (untreated) B. 20 µg Huh7 whole cell lysate/extract (3uM-Thapsipargin treatment for 12hr)0 % SDS-PAGESQSTM1 antibody [N3C1], Internal (GTX100685) dilution: 1:1500
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract B. 30 ?g Rat2 whole cell lysate/extract10% SDS-PAGESQSTM1 antibody [N3C1], Internal (GTX100685) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SQSTM1 antibody detects SQSTM1 protein by western blot analysis. Whole cell extracts (30 ?g) prepared from Huh7 cells treated with Thapsigargin for 12 or 24 hours or untreated (-) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (GTX100685) at a dilution of 1:1000.The Beta Actin was used as internal control (GTX110564, 1:20000) shown at the bottom panel.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) Huh-7 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) HepG2 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HepG2 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) HepG2 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.Samples: HepG2 cells treated with 3£gM thapsigargin 12 hrs (rigtht) and mock (left) were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acetone for 1 min .Green: SQSTM1 protein stained by SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50£gM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: SQSTM1 protein stained by SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:1000.Red: Phalloidin, a F-actin marker.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of SQSTM1 protein from HeLa whole cell extracts using 5 £gg of SQSTM1 antibody [N3C1], Internal (GTX100685).Western blot analysis was performed using SQSTM1 antibody [N3C1], Internal (GTX100685).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SQSTM1 antibody [N3C1], Internal (GTX100685) detects SQSTM1 protein by flow cytometry analysis.Sample: HeLa cell fixed in 4% paraformaldehyde at 4?C for 5 min.Brown: Unlabelled sample was also used as a control.Blue: SQSTM1 antibody [N3C1], Internal] (GTX100685) dilution: 1:100.Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.

GTX100685

Antibody data