F48010

antibody from NSJ Bioreagents

Targeting: SQSTM1 A170, OSIL, p60, p62, p62B, PDB3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

F48010

Provider

NSJ Bioreagents

Product name

P62 Antibody

Antibody type

Polyclonal

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of p62 antibody at 1:500 dilution + HeLa lysate; Predicted band size : 47~62 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of p62 antibody pre-incubated without (Lane 1) and with (2) blocking peptide in MCF-7 lysate

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of p6 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SQSTM1/p62 gene (2).

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Fluorescent image of U251 cells stained with p62 antibody at 1:200. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescence staining of p62 antibody on Methanol-fixed and PFA fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki, Finland.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC analysis of FFPE human hepatocarcinoma tissue stained with the p62 antibody