M00300-1
antibody from Boster Biological Technology
Targeting: SQSTM1
A170, OSIL, p60, p62, p62B, PDB3
Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- M00300-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-SQSTM1/p62 Antibody Picoband™ (monoclonal, 3H11)
- Antibody type
- Monoclonal
- Description
- Mouse IgG monoclonal antibody for SQSTM1/p62 detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3H11
- Vial size
- 100μg/vial
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Submitted references LncRNA THRIL, transcriptionally activated by AP-1 and stabilized by METTL14-mediated m6A modification, accelerates LPS-evoked acute injury in alveolar epithelial cells.
Radiosensitizer EXO-miR-197-3p Inhibits Nasopharyngeal Carcinoma Progression and Radioresistance by Regulating the AKT/mTOR Axis and HSPA5-mediated Autophagy.
Generation and Characterization of Novel Monoclonal Antibodies Targeting p62/sequestosome-1 Across Human Neurodegenerative Diseases.
Shi Q, Li Z, Dong Y, Yang G, Li M
International immunopharmacology 2023 Oct;123:110740
International immunopharmacology 2023 Oct;123:110740
Radiosensitizer EXO-miR-197-3p Inhibits Nasopharyngeal Carcinoma Progression and Radioresistance by Regulating the AKT/mTOR Axis and HSPA5-mediated Autophagy.
Jiang J, Tang Q, Gong J, Jiang W, Chen Y, Zhou Q, Aldeen A, Wang S, Li C, Lv W, Du T, Wang X, Long X, Feng X
International journal of biological sciences 2022;18(5):1878-1895
International journal of biological sciences 2022;18(5):1878-1895
Generation and Characterization of Novel Monoclonal Antibodies Targeting p62/sequestosome-1 Across Human Neurodegenerative Diseases.
Trejo-Lopez JA, Sorrentino ZA, Riffe CJ, Prokop S, Dickson DW, Yachnis AT, Giasson BI
Journal of neuropathology and experimental neurology 2020 Apr 1;79(4):407-418
Journal of neuropathology and experimental neurology 2020 Apr 1;79(4):407-418
No comments: Submit comment
Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of SQSTM1 using anti-SQSTM1 antibody (M00300-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CCRF-CEM whole cell lysates Lane 2: human HepG2 whole cell lysates Lane 3: human HT1080 whole cell lysates Lane 4: human SW620 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SQSTM1 antigen affinity purified monoclonal antibody (Catalog # M00300-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SQSTM1 at approximately 62KD. The expected band size for SQSTM1 is at 62KD.
- Additional image